Role of flow cytometry in pediatric hematopathology. Am J Clin Pathol 2004 Dec;122 Suppl:S19-32
Date
02/05/2005Pubmed ID
15690640DOI
10.1309/1GLC0VY081X8HY2VScopus ID
2-s2.0-13844254426 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
Multiparameter flow cytometry with optimally selected antibody combinations has expanded the use of this technique beyond traditional applications in hematopathology. By analyzing qualitative patterns of antigen expression on discrete populations or "clusters," one can detect immunophenotypic aberrancy in specific cell populations relative to normal and reactive populations. Evaluation of patterns of antigen expression can also be used to supplement conventional methodologies in the diagnosis and subclassification of certain types of hematologic neoplasia. Finally, the diagnosis of some congenital disorders affecting the hematolymphoid system can be facilitated by the detection of characteristic immunophenotypic changes. Examples of applications of multiparameter flow cytometry with particular relevance to pediatric hematopathology are discussed.
Author List
Kroft SHAuthor
Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Burkitt LymphomaChild
Down Syndrome
Flow Cytometry
Genotype
Hematologic Diseases
Hematologic Neoplasms
Humans
Image Cytometry
Immunophenotyping
Leukemia-Lymphoma, Adult T-Cell
Lymphoma
