Faculty Collaboration Database

Characterization of B-cell subpopulations in patients with chronic rhinosinusitis. Int Forum Allergy Rhinol 2013 Aug;3(8):621-9

Date

05/04/2013

Pubmed ID

23640795

DOI

10.1002/alr.21173

Scopus ID

2-s2.0-84882271743 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

BACKGROUND: Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRS patients.

METHODS: Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single-cell suspension flow cytometry these samples were analyzed for overall and stage-specific B and plasma cell percentages.

RESULTS: Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells.

CONCLUSION: This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.

Author List

Psaltis AJ, Schlosser RJ, Yawn JR, Henriquez O, Mulligan JK

Author

James Roy Yawn MD Assistant Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
B-Lymphocyte Subsets
B-Lymphocytes
Case-Control Studies
Chronic Disease
Female
Flow Cytometry
Humans
Male
Middle Aged
Nasal Mucosa
Nasal Polyps
Paranasal Sinuses
Plasma Cells
Rhinitis
Sinusitis