Faculty Collaboration Database

Stimulation of inorganic pyrophosphate elaboration by cultured cartilage and chondrocytes. Arch Biochem Biophys 1989 Aug 01;272(2):393-9

Date

08/01/1989

Pubmed ID

2546499

DOI

10.1016/0003-9861(89)90233-6

Scopus ID

2-s2.0-0024384618 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

Inorganic pyrophosphate elaboration by articular cartilage may favor calcium pyrophosphate dihydrate crystal deposition. Frequently crystal deposits form in persons affected with metabolic diseases. The cartilage organ culture system was used to model these metabolic conditions while measuring the influence on extracellular pyrophosphate elaboration. Alterations of ambient pH, thyroid stimulating hormone levels, and parathyroid hormone levels did not change pyrophosphate accumulation in the media. However, subphysiologic ambient calcium concentrations (25, 100, 500 microM) increased pyrophosphate accumulation about chondrocytes 3- to 10-fold. Low calcium also induced release of [14C]adenine-labeled nucleotides from chondrocytes, potential substrates for generation of extracellular pyrophosphate by ectoenzymes. Exposing cartilage to 10% fetal bovine serum also enhanced by 50% the egress of inorganic pyrophosphate from the tissue.

Author List

Ryan LM, Kurup I, Rosenthal AK, McCarty DJ

Author

Ann K. Rosenthal MD Associate Dean, Chief, Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Calcium
Cartilage, Articular
Chondrocalcinosis
Diphosphates
Hydrogen-Ion Concentration
In Vitro Techniques
Iron
Parathyroid Hormone
Pyrophosphatases
Sulfates
Swine
Thyrotropin