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Production of hydroxyeicosatetraenoic acids and prostaglandins by a novel rat microglial cell line. J Neuroimmunol 2004 Apr;149(1-2):130-41

Date

03/17/2004

Pubmed ID

15020073

DOI

10.1016/j.jneuroim.2003.12.022

Scopus ID

2-s2.0-12144287853 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.

Author List

Rademacher DJ, Kearn CS, Carrier EJ, Patel S, Delgado MA, Barkmeier A, Klick DE, Breese NM, Pfister SL, Nithipatikom K, Campbell WB, Hillard CJ

Authors

William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
Cecilia J. Hillard PhD Associate Dean, Center Director, Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
Sandra L. Pfister PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Newborn
Blotting, Western
Cell Differentiation
Cell Line
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Enzyme Inhibitors
Enzyme-Linked Immunosorbent Assay
Glial Fibrillary Acidic Protein
Hydroxyeicosatetraenoic Acids
Immunohistochemistry
Lectins
Macrophage Colony-Stimulating Factor
Mass Spectrometry
Microglia
Prostaglandin-Endoperoxide Synthases
Prostaglandins
Rats
Rats, Sprague-Dawley
Thymidine
Time Factors
Tritium
Tumor Necrosis Factor-alpha