Medical College of Wisconsin
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roundabout4 is essential for angiogenesis in vivo. Proc Natl Acad Sci U S A 2005 May 03;102(18):6373-8

Date

04/26/2005

Pubmed ID

15849270

Pubmed Central ID

PMC1088354

DOI

10.1073/pnas.0408318102

Abstract

Stereotypical patterns of vascular and neuronal networks suggest that specific genetic programs tightly control path determination and, consequently, angiogenesis and axon-guidance mechanisms. Our study focuses on one member of the roundabout family of receptors, which traditionally mediate repulsion from the midline. Here, we characterize a fourth member of this family, roundabout4 (robo4), which is the predominant roundabout (robo) that is expressed in embryonic zebrafish vasculature. Gene knockdown and overexpression approaches show that robo4 is essential for coordinated symmetric and directed sprouting of intersomitic vessels and provide mechanistic insights into this process. Also, human robo4 gene functionally compensates for loss of robo4 gene function, suggesting evolutionary conservation. This article reports an endothelial-specific function for a robo gene in vertebrates in vivo.

Author List

Bedell VM, Yeo SY, Park KW, Chung J, Seth P, Shivalingappa V, Zhao J, Obara T, Sukhatme VP, Drummond IA, Li DY, Ramchandran R

Author

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
Cloning, Molecular
Computational Biology
DNA Primers
Endothelium
Gene Expression
Genetic Complementation Test
Immunohistochemistry
In Situ Hybridization
Molecular Sequence Data
Neovascularization, Physiologic
Oligonucleotides
Receptors, Cell Surface
Sequence Homology
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d