TGF-beta 2 reduces demyelination, virus antigen expression, and macrophage recruitment in a viral model of multiple sclerosis. J Immunol 2000 Mar 15;164(6):3207-13
Date
03/08/2000Pubmed ID
10706712DOI
10.4049/jimmunol.164.6.3207Scopus ID
2-s2.0-0034653933 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
TGF-beta 2 is a potent immunoregulatory mediator that influences B cell, T cell, and macrophage function. To test whether this cytokine alters pathology in a model of virus-induced demyelinating disease, we treated SJL/J mice with TGF-beta 2 either before or after infection with Theiler's murine encephalomyelitis virus. Treatment continued three times weekly through day 35 postinfection. TGF-beta 2 administration resulted in significantly smaller lesions and fewer virus Ag-positive cells in the spinal cords of infected SJL/J mice. Mice treated with TGF-beta 2 had similar levels of virus-specific IgG as infected, control-treated mice. TGF-beta 2 administration significantly increased the level of non-virus-specific activated CTLs, but had no effect on virus-specific CTLs. TUNEL revealed a decrease in the number of apoptotic nuclei in the spinal cord white matter of mice treated in vivo with TGF-beta 2. Immunostaining with an Ab to F4/80 revealed that TGF-beta 2-treated mice had significantly fewer F4/80-positive cells in the white matter of the spinal cord as compared with infected control-treated mice. These data suggest that TGF-beta 2 may control virus-induced demyelination via an immunomodulatory mechanism that reduces macrophage infiltration.
Author List
Drescher KM, Murray PD, Lin X, Carlino JA, Rodriguez MAuthor
Paul D. Harker-Murray MD, PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Viral
Apoptosis
Cardiovirus Infections
Cell Count
Cytotoxicity Tests, Immunologic
Disease Models, Animal
Disease Susceptibility
Female
Injections, Intraperitoneal
Macrophages
Mice
Mice, Inbred Strains
Multiple Sclerosis
Myelin Sheath
Spinal Cord
T-Lymphocytes, Cytotoxic
Theilovirus
Transforming Growth Factor beta
Viral Plaque Assay
Virus Replication
