Mmp17b is essential for proper neural crest cell migration in vivo. PLoS One 2013;8(10):e76484
Date
10/08/2013Pubmed ID
24098510Pubmed Central ID
PMC3788140DOI
10.1371/journal.pone.0076484Scopus ID
2-s2.0-84885036837 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b function, or chemical inhibition of MMP activity results in aberrant NC cell migration with minimal change in NC proliferation or apoptosis. Intriguingly, a GPI anchored protein with metalloproteinase inhibitor properties, Reversion-inducing-Cysteine-rich protein with Kazal motifs (RECK), which has previously been implicated in NC development, is expressed in close apposition to NC cells expressing mmp17b, raising the possibility that these two gene products interact. Consistent with this possibility, embryos silenced for mmp17b show defective development of the dorsal root ganglia (DRG), a crest-derived structure affected in RECK mutant fish sensory deprived (sdp). Taken together, this study has identified the first pair of MMP, and their putative MMP inhibitor RECK that functions together in NC cell migration.
Author List
Leigh NR, Schupp MO, Li K, Padmanabhan V, Gastonguay A, Wang L, Chun CZ, Wilkinson GA, Ramchandran RAuthors
Keguo Li MD, PhD Research Scientist I in the Surgery department at Medical College of WisconsinRamani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Body Patterning
Cell Movement
Embryonic Development
Enzyme Activation
GPI-Linked Proteins
Gene Expression Profiling
Matrix Metalloproteinase 17
Molecular Sequence Data
Neural Crest
Organ Specificity
Sequence Alignment
Zebrafish
