Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions. Exp Cell Res 2005 Nov 01;310(2):383-91
Date
09/20/2005Pubmed ID
16168985DOI
10.1016/j.yexcr.2005.08.015Scopus ID
2-s2.0-26844431677 (requires institutional sign-in at Scopus site) 47 CitationsAbstract
Several recent reports suggest that there is far more plasticity that previously believed in the developmental potential of bone-marrow-derived cells (BMCs) that can be induced by extracellular developmental signals of other lineages whose nature is still largely unknown. In this study, we demonstrate that bone-marrow-derived mesenchymal stem cells (MSCs) co-cultured with mouse proliferating or fixed (by paraformaldehyde or methanol) neural stem cells (NSCs) generate neural stem cell-like cells with a higher expression of Sox-2 and nestin when grown in NS-A medium supplemented with N2, NSC conditioned medium (NSCcm) and bFGF. These neurally induced MSCs eventually differentiate into beta-III-tubulin and GFAP expressing cells with neuronal and glial morphology when grown an additional week in Neurobasal/B27 without bFGF. We conclude that juxtacrine interaction between NSCs and MSCs combined with soluble factors released from NSCs are important for generation of neural-like cells from bone-marrow-derived adherent MSCs.
Author List
Alexanian ARAuthor
Arshak R. Alexanian VMD, PhD Adjunct Associate Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBone Marrow Cells
Cell Differentiation
Cells, Cultured
Coculture Techniques
DNA-Binding Proteins
Female
Intermediate Filament Proteins
Male
Mice
Mice, Inbred Strains
Nerve Tissue Proteins
Nestin
Neurons
Paracrine Communication
SOXB1 Transcription Factors
Stem Cells
Trans-Activators
