Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites. Anal Biochem 2006 May 01;352(1):129-34
Date
02/17/2006Pubmed ID
16480681DOI
10.1016/j.ab.2006.01.005Scopus ID
2-s2.0-33646065988 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
An HPLC method for the chiral analysis of the four regioisomeric epoxyeicosatrienoic acids (EETs) is described. The cytochrome P450 arachidonic acid epoxygenase metabolites are resolved, without the need for derivatization, by chiral-phase HPLC on a Chiralcel OJ column. Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase.
Author List
Wei S, Brittin JJ, Falck JR, Anjaiah S, Nithipatikom K, Cui L, Campbell WB, Capdevila JHAuthor
William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
8,11,14-Eicosatrienoic AcidAnimals
Chromatography, High Pressure Liquid
Cytochrome P-450 Enzyme System
Liver
Male
Microsomes, Liver
Oxygenases
Rats
Rats, Sprague-Dawley
Stereoisomerism
Vasodilator Agents
