Methylation-associated silencing of microRNA-34b in hepatocellular carcinoma cancer. Gene 2014 Jun 10;543(1):101-7
Date
04/08/2014Pubmed ID
24704024DOI
10.1016/j.gene.2014.03.059Scopus ID
2-s2.0-84899646114 (requires institutional sign-in at Scopus site) 53 CitationsAbstract
MicroRNAs (miRNAs) can act as oncogenes or tumor-suppressor genes in human cancers including HCC. Previous studies have identified miR-34 family as an important component of the tumor suppressor network during carcinogenesis. In this study, we investigated the methylation status of miR-34 family in HCC tumor and adjacent non-tumor tissues using methylation-specific PCR (MSP). The methylation frequencies of miR-34a and miR-34b/c were 72.1% (31/43) and 79.1% (34/43) in HCC tissues, which were significantly higher than that in the adjacent non-tumor tissues (P < 0.05), respectively. The results were validated by bisulfite sequencing PCR (BSP). Quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis showed that the expression of miR-34a and miR-34b was significantly down-regulated in HCC tissues compared with adjacent non-tumor tissues (P < 0.05). Moreover, the expression of miR-34b was inversely correlated to CpG island methylation in tumor tissues, but not for miR-34a. In summary, our results suggest that DNA methylation may be involved in the inactivation of miR-34b in HCC.
Author List
Xie K, Liu J, Chen J, Dong J, Ma H, Liu Y, Hu ZAuthor
Jing Dong PhD Assistant Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAged
Aged, 80 and over
Carcinoma, Hepatocellular
CpG Islands
DNA Methylation
Female
Gene Expression Regulation, Neoplastic
Gene Silencing
Humans
Liver Neoplasms
Male
MicroRNAs
Middle Aged
