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De novo expression of sodium-glucose cotransporter SGLT2 in Bowman's capsule coincides with replacement of parietal epithelial cell layer with proximal tubule-like epithelium. J Membr Biol 2014 Aug;247(8):675-83

Date

06/08/2014

Pubmed ID

24906870

Pubmed Central ID

PMC4117812

DOI

10.1007/s00232-014-9686-4

Scopus ID

2-s2.0-84905576038   4 Citations

Abstract

In kidney nephron, parietal epithelial cells line the Bowman's capsule and function as a permeability barrier for the glomerular filtrate. Bowman's capsule cells with proximal tubule epithelial morphology have been found. However, the effects of tubular metaplasia in Bowman's capsule on kidney function remain poorly understood. Sodium-glucose cotransporter 2 (SGLT2) plays a major role in reabsorption of glucose in the kidney and is expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule. We hypothesized that SGLT2 is expressed in tubularized Bowman's capsule and used our novel antibody to test this hypothesis. Immunohistochemical analysis was performed with our SGLT2 antibody on C57BL/6 mouse kidney prone to have tubularized Bowman's capsules. Cell membrane was examined with periodic acid-Schiff (PAS) stain. The results showed that SGLT2 was localized on BBM of the proximal tubules in young and adult mice. Bowman's capsules were lined mostly with normal brush border-less parietal epithelial cells in young mice, while they were almost completely covered with proximal tubule-like cells in adult mice. Regardless of age, SGLT2 was expressed on BBM of the tubularized Bowman's capsule but did not co-localize with nephrin in the glomerulus. SGLT2-expressing tubular cells expanded from the urinary pole toward the vascular pole of the Bowman's capsule. This study identified the localization of SGLT2 in the Bowman's capsule. Bowman's capsules with tubular metaplasia may acquire roles in reabsorption of filtered glucose and sodium.

Author List

Tabatabai NM, North PE, Regner KR, Kumar SN, Duris CB, Blodgett AB

Authors

Suresh Kumar PhD Associate Professor in the Pathology department at Medical College of Wisconsin
Paula E. North MD, PhD Professor in the Pathology department at Medical College of Wisconsin
Kevin R. Regner MD Chief, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Blotting, Western
Bowman Capsule
Cell Membrane
Epithelium
Glucose
Immunoenzyme Techniques
Kidney
Kidney Tubules, Proximal
Male
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Microvilli
Sodium
Sodium-Glucose Transporter 2
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a