Differential response of gel-forming mucins to pathogenic middle ear bacteria. Int J Pediatr Otorhinolaryngol 2014 Aug;78(8):1368-73
Date
06/25/2014Pubmed ID
24958163Pubmed Central ID
PMC4104932DOI
10.1016/j.ijporl.2014.05.037Scopus ID
2-s2.0-84904036054 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
OBJECTIVE: To assess the differential response of the secretory gel forming mucins (GFM) to the most common bacterial pathogens causing otitis media, Streptococcus pneumoniae (SP), nontypeable Haemophilus influenza (NTHi), and Moraxella catarrhalis (Mcat), in a culture model of human middle ear epithelium (HMEEC).
METHODS: In vitro cultured HMEEC was exposed to 5 μg/ml of bacterial whole cell lysate (WCL). RNA was extracted to generate cDNA. The expression levels of each of the targeted mucin transcripts, MUC2, MUC5AC, MUC5B and MUC19, were detected by quantitative PCR.
RESULTS: The submerged HMEEC exposed to NTHi-86028NP WCL demonstrated a significant increase of MUC2, MUC5AC and MUC5B as compared to the control non-treated cells while MUC19 transcript level remained unchanged. WCL of additional major OM pathogens significantly increase the transcription of these three mucin genes as well. A combination of NTHi and SP further synergistically induced MUC2 and MUC5AC gene expression however, not all NTHi strains synergized with SP in the induction. Addition of Mcat WCL to the synergized combination of NTHi and SP did not participate in the synergistic response of mucins.
CONCLUSION: The specific pathogen combinations were important in determining the degree of synergistic effects to GFM expression. The current data are substantive in guiding future work to extend our understanding of OM pathogens and GFMs.
Author List
Kerschner JE, Hong W, Khampang P, Johnston NAuthors
Wenzhou Hong DVM, PhD Assistant Professor in the Otolaryngology department at Medical College of WisconsinNikki Johnston PhD Professor in the Otolaryngology department at Medical College of Wisconsin
Joseph E. Kerschner MD Provost, Executive Vice President, Dean, Professor in the School of Medicine Administration department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Bacterial ProteinsCells, Cultured
Ear, Middle
Epithelial Cells
Gels
Haemophilus influenzae
Humans
Mucins
Otitis Media
Polymerase Chain Reaction
Streptococcus pneumoniae
Transcription, Genetic
