Association between risk of oral precancer and genetic variations in microRNA and related processing genes. J Biomed Sci 2014 May 17;21(1):48
Date
06/03/2014Pubmed ID
24885463Pubmed Central ID
PMC4035900DOI
10.1186/1423-0127-21-48Scopus ID
2-s2.0-84901688781 (requires institutional sign-in at Scopus site) 17 CitationsAbstract
BACKGROUND: MicroRNAs have been implicated in cancer but studies on their role in precancer, such as leukoplakia, are limited. Sequence variations at eight miRNA and four miRNA processing genes were studied in 452 healthy controls and 299 leukoplakia patients to estimate risk of disease.
RESULTS: Genotyping by TaqMan assay followed by statistical analyses showed that variant genotypes at Gemin3 and mir-34b reduced risk of disease [OR = 0.5(0.3-0.9) and OR = 0.7(0.5-0.9) respectively] in overall patients as well as in smokers [OR = 0.58(0.3-1) and OR = 0.68(0.5-0.9) respectively]. Among chewers, only mir29a significantly increased risk of disease [OR = 1.8(1-3)]. Gene-environment interactions using MDR-pt program revealed that mir29a, mir34b, mir423 and Xpo5 modulated risk of disease (p < 0.002) which may be related to change in expression of these genes as observed by Real-Time PCR assays. But association between polymorphisms and gene expressions was not found in our sample set as well as in larger datasets from open access platforms like Genevar and 1000 Genome database.
CONCLUSION: Variations in microRNAs and their processing genes modulated risk of precancer but further in-depth study is needed to understand mechanism of disease process.
Author List
Roy R, De Sarkar N, Ghose S, Paul RR, Ray A, Mukhopadhyay I, Roy BAuthor
Navonil De Sarkar PhD Assistant Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultFemale
Gene-Environment Interaction
Genetic Association Studies
Genetic Predisposition to Disease
Genotype
Humans
Leukoplakia
Male
MicroRNAs
Middle Aged
Polymorphism, Single Nucleotide
Risk Factors
