Cardioprotection from ischemia-reperfusion injury by near-infrared light in rats. Photomed Laser Surg 2014 Sep;32(9):505-11
Date
08/06/2014Pubmed ID
25093393Pubmed Central ID
PMC4142831DOI
10.1089/pho.2014.3743Scopus ID
2-s2.0-84906490690 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
UNLABELLED: Abstract Objective: Myocardial reperfusion injury can induce further cardiomyocyte death and contribute to adverse cardiovascular outcomes after myocardial ischemia, cardiac surgery, or circulatory arrest. Exposure to near-infrared (NIR) light at the time of reoxygenation protects neonatal rat cardiomyocytes and HL-1 cells from injury. We hypothesized that application of NIR at 670 nm would protect the heart against ischemia-reperfusion injury.
METHODS: We assessed the protective role of NIR in in vivo and in vitro rat models of ischemia-reperfusion injury.
RESULTS: NIR application had no effect on the function of the nonischemic isolated heart, and had no effect on infarct size when applied during global ischemia. In the in vivo model, NIR commencing immediately before reperfusion decreased infarct size by 40%, 33%, 38%, and 77%, respectively, after regional ischemic periods of 30, 20, 15, and 10 min. Serum cardiac troponin I (cTnI) was significantly reduced in the 15 min group, whereas creatine kinase (CK) and lactate dehydrogenase (LDH) levels were not affected.
CONCLUSIONS: We have demonstrated the safety of NIR application in an in vitro rat isolated model. In addition, we have demonstrated safety and efficacy when using NIR for cardioprotection in an in vivo rat ischemia model, and that this cardioprotection is dependent upon some factor present in blood, but not in perfusion buffer. RESULTS show potential for cTnI, but not CK or LDH, as a biomarker for cardioprotection by NIR. NIR may have therapeutic utility in providing myocardial protection from ischemia-reperfusion injury.
Author List
Quirk BJ, Sonowal P, Jazayeri MA, Baker JE, Whelan HTAuthor
John E. Baker PhD Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBiomarkers
Disease Models, Animal
Heart
In Vitro Techniques
Infrared Rays
Male
Myocardial Reperfusion Injury
Rats
Rats, Sprague-Dawley