Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Cardioprotection from ischemia-reperfusion injury by near-infrared light in rats. Photomed Laser Surg 2014 Sep;32(9):505-11



Pubmed ID


Pubmed Central ID




Scopus ID

2-s2.0-84906490690   5 Citations


UNLABELLED: Abstract Objective: Myocardial reperfusion injury can induce further cardiomyocyte death and contribute to adverse cardiovascular outcomes after myocardial ischemia, cardiac surgery, or circulatory arrest. Exposure to near-infrared (NIR) light at the time of reoxygenation protects neonatal rat cardiomyocytes and HL-1 cells from injury. We hypothesized that application of NIR at 670 nm would protect the heart against ischemia-reperfusion injury.

METHODS: We assessed the protective role of NIR in in vivo and in vitro rat models of ischemia-reperfusion injury.

RESULTS: NIR application had no effect on the function of the nonischemic isolated heart, and had no effect on infarct size when applied during global ischemia. In the in vivo model, NIR commencing immediately before reperfusion decreased infarct size by 40%, 33%, 38%, and 77%, respectively, after regional ischemic periods of 30, 20, 15, and 10 min. Serum cardiac troponin I (cTnI) was significantly reduced in the 15 min group, whereas creatine kinase (CK) and lactate dehydrogenase (LDH) levels were not affected.

CONCLUSIONS: We have demonstrated the safety of NIR application in an in vitro rat isolated model. In addition, we have demonstrated safety and efficacy when using NIR for cardioprotection in an in vivo rat ischemia model, and that this cardioprotection is dependent upon some factor present in blood, but not in perfusion buffer. RESULTS show potential for cTnI, but not CK or LDH, as a biomarker for cardioprotection by NIR. NIR may have therapeutic utility in providing myocardial protection from ischemia-reperfusion injury.

Author List

Quirk BJ, Sonowal P, Jazayeri MA, Baker JE, Whelan HT


John E. Baker PhD Professor in the Surgery department at Medical College of Wisconsin
Harry T. Whelan MD Professor in the Neurology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Disease Models, Animal
In Vitro Techniques
Infrared Rays
Myocardial Reperfusion Injury
Rats, Sprague-Dawley
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a