Faculty Collaboration Database

Smooth muscle-protein translocation and tissue function. Anat Rec (Hoboken) 2014 Sep;297(9):1734-46

Date

08/16/2014

Pubmed ID

25125185

Pubmed Central ID

PMC4244760

DOI

10.1002/ar.22970

Scopus ID

2-s2.0-84906096995 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

Smooth muscle (SM) tissue is a complex organization of multiple cell types and is regulated by numerous signaling molecules (neurotransmitters, hormones, cytokines, etc.). SM contractile function can be regulated via expression and distribution of the contractile and cytoskeletal proteins, and activation of any of the second messenger pathways that regulate them. Spatial-temporal changes in the contractile, cytoskeletal or regulatory components of SM cells (SMCs) have been proposed to alter SM contractile activity. Ca(2+) sensitization/desensitization can occur as a result of changes at any of these levels, and specific pathways have been identified at all of these levels. Understanding when and how proteins can translocate within the cytoplasm, or to-and-from the plasmalemma and the cytoplasm to alter contractile activity is critical. Numerous studies have reported translocation of proteins associated with the adherens junction and G protein-coupled receptor activation pathways in isolated SMC systems. Specific examples of translocation of vinculin to and from the adherens junction and protein kinase C (PKC) and 17 kDa PKC-potentiated inhibitor of myosin light chain phosphatase (CPI-17) to and from the plasmalemma in isolated SMC systems but not in intact SM tissues are discussed. Using both isolated SMC systems and SM tissues in parallel to pursue these studies will advance our understanding of both the role and mechanism of these pathways as well as their possible significance for Ca(2+) sensitization in intact SM tissues and organ systems.

Author List

Eddinger TJ

Author

Thomas Eddinger PhD Bioological Sciences in the Biology department at Marquette University

MESH terms used to index this publication - Major topics in bold

Adherens Junctions
Animals
Calcium
Excitation Contraction Coupling
Humans
Intracellular Signaling Peptides and Proteins
Muscle Contraction
Muscle Proteins
Muscle, Smooth
Myocytes, Smooth Muscle
Phosphoprotein Phosphatases
Protein Kinase C
Protein Transport
Receptors, G-Protein-Coupled
Signal Transduction
Time Factors
Vinculin