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Analysis of aPKClambda and aPKCzeta reveals multiple and redundant functions during vertebrate retinogenesis. Mol Cell Neurosci 2007 Mar;34(3):431-44

Date

01/16/2007

Pubmed ID

17223574

Pubmed Central ID

PMC2700298

DOI

10.1016/j.mcn.2006.11.016

Scopus ID

2-s2.0-33847311778   36 Citations

Abstract

Retinal lamination is known to depend on cell polarity and localized signaling. In vertebrates, atypical protein kinase C proteins, aPKClambda/iota and aPKCzeta, are essential for apical-basal cell polarity. However, it is not known to what extent functional redundancy has precluded a comprehensive functional characterization of aPKC signaling during vertebrate retinogenesis. Here, we show that aPKCs lambda and zeta are functionally redundant for multiple aspects of retinogenesis including mitotic division location and orientation, cell-type positioning, and retinal pigment epithelial (RPE) and photoreceptor cell morphogenesis. Genetic mosaic analyses demonstrate a cell-autonomous requirement of aPKCs for RPE and photoreceptor development, and a cell-non-cell-autonomous function that is intrinsic to the neural retina for cell-type positioning. Our observations uncover a previously unappreciated involvement of aPKCzeta during zebrafish retinogenesis and suggest that aPKC signaling primes the retinal environment for appropriate cell migration of post-mitotic progenitor cells but is not essential for correct cell-type specification.

Author List

Cui S, Otten C, Rohr S, Abdelilah-Seyfried S, Link BA

Author

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bromodeoxyuridine
Cell Count
Cell Differentiation
Cell Polarity
Cell Proliferation
Embryo, Nonmammalian
Eye Proteins
Gene Expression Regulation, Developmental
Immunohistochemistry
Isoenzymes
Morphogenesis
Protein Kinase C
Retina
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-486 e3098984f26de787f5ecab75090d0a28e7f4f7c0