cCMP and cUMP occur in vivo. Biochem Biophys Res Commun 2015 May 15;460(4):909-14
Date
04/04/2015Pubmed ID
25838203Pubmed Central ID
PMC4765920DOI
10.1016/j.bbrc.2015.03.115Scopus ID
2-s2.0-84937760629 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
Mammalian cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. It is unknown whether these tentative new second messenger molecules occur in vivo. We used high performance liquid chromatography quadrupole tandem mass spectrometry to quantitate nucleoside 3',5'-cyclic monophosphates. cCMP was detected in all organs studied, most notably pancreas, spleen and the female reproductive system. cUMP was not detected in organs, probably due to the intrinsically low sensitivity of mass spectrometry to detect this molecule and organ matrix effects. Intratracheal infection of mice with recombinant Pseudomonas aeruginosa harboring the nucleotidyl cyclase toxin ExoY massively increased cUMP in lung. The identity of cCMP and cUMP in organs was confirmed by high performance liquid chromatography quadrupole time of flight mass spectrometry. cUMP also appeared in serum, urine and faeces following infection. Taken together, this report unequivocally shows for the first time that cCMP and cUMP occur in vivo.
Author List
Bähre H, Hartwig C, Munder A, Wolter S, Stelzer T, Schirmer B, Beckert U, Frank DW, Tümmler B, Kaever V, Seifert RAuthor
Dara W. Frank PhD Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsChromatography, High Pressure Liquid
Cyclic CMP
Female
Mice
Mice, Inbred C57BL
Nucleotides, Cyclic
Real-Time Polymerase Chain Reaction
Tandem Mass Spectrometry
Uridine Monophosphate
