Activation of 5-HT1A receptors in the preBötzinger region has little impact on the respiratory pattern. Respir Physiol Neurobiol 2015 Jul;212-214:9-19
Date
04/08/2015Pubmed ID
25850079DOI
10.1016/j.resp.2015.03.005Scopus ID
2-s2.0-84928340110 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
The preBötzinger (preBötC) complex has been suggested as the primary site where systemically administered selective serotonin agonists have been shown to reduce or prevent opioid-induced depression of breathing. However, this hypothesis has not been tested pharmacologically in vivo. This study sought to determine whether 5-HT1A receptors within the preBötC and ventral respiratory column (VRC) mediate the tachypneic response induced by intravenous (IV) (±)-8-Hydroxy-2-diproplyaminotetralin hydrobromide (8-OH-DPAT) in a decerebrated dog model. IV 8-OH-DPAT (19 ± 2 μg/kg) reduced both inspiratory (I) and expiratory (E) durations by ∼ 40%, but had no effect on peak phrenic activity (PPA). Picoejection of 1, 10, and 100 μM 8-OH-DPAT on I and E preBötC neurons produced dose-dependent decreases up to ∼ 40% in peak discharge. Surprisingly, microinjections of 8-OH-DPAT and 5-HT within the VRC from the obex to 9 mm rostral had no effect on timing and PPA. These results suggest that the tachypneic effects of IV 8-OH-DPAT are due to receptors located outside of the areas we studied.
Author List
Radocaj T, Mustapic S, Prkic I, Stucke AG, Hopp FA, Stuth EA, Zuperku EJAuthors
Astrid G. Stucke MD Professor in the Anesthesiology department at Medical College of WisconsinEckehard A. Stuth MD Professor in the Anesthesiology department at Medical College of Wisconsin
Edward J. Zuperku PhD Professor in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
8-Hydroxy-2-(di-n-propylamino)tetralinAction Potentials
Analysis of Variance
Animals
Disease Models, Animal
Dogs
Dose-Response Relationship, Drug
Female
Male
Medulla Oblongata
Microinjections
Neurons
Phrenic Nerve
Receptor, Serotonin, 5-HT1A
Respiration
Serotonin
Serotonin Receptor Agonists
Tachypnea
