Cloning of the gene encoding rat JAK2, a protein tyrosine kinase. Gene 1995 Jun 09;158(2):281-5
Date
06/09/1995Pubmed ID
7607555DOI
10.1016/0378-1119(95)00041-4Scopus ID
2-s2.0-0029059915 (requires institutional sign-in at Scopus site) 27 CitationsAbstract
A complete cDNA clone encoding the rat JAK2 protein tyrosine kinase was isolated from an Nb2-SP (rat pre-T lymphoma cell line) cDNA library. The nucleotide (nt) and deduced amino acid (aa) sequences for this clone were determined and an open reading frame of 3399 bp, encoding a protein of a deduced mass of 130 kDa, was found. The coding regions of the rat and murine Jak2 clones share 93.4% nt identity and 97.1% aa identity. Northern analysis demonstrated that the 5-kb mRNA is highly abundant in brain and spleen, less abundant in skeletal muscle and testis, and detectable in kidney, heart, lung and liver. Translation of the rat Jak2 mRNA in rabbit reticulocytes results in a protein which is specifically immunoprecipitated by antibodies (Ab) recognizing JAK2, but not by Ab recognizing JAK1.
Author List
Duhé RJ, Rui H, Greenwood JD, Garvey K, Farrar WLMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Base Sequence
Blotting, Northern
Conserved Sequence
DNA, Complementary
Gene Library
Janus Kinase 2
Lymphoma
Molecular Sequence Data
Protein Biosynthesis
Protein-Tyrosine Kinases
Proto-Oncogene Proteins
Rats
Sequence Analysis, DNA
Tissue Distribution
Transcription, Genetic
Tumor Cells, Cultured
