AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods Mol Biol 2016;1382:251-61
Date
11/28/2015Pubmed ID
26611592Pubmed Central ID
PMC5459312DOI
10.1007/978-1-4939-3271-9_18Scopus ID
2-s2.0-84948768833 (requires institutional sign-in at Scopus site) 19 CitationsAbstract
Transferring genetic molecules into the peripheral sensory nervous system to manipulate nociceptive pathophysiology is a powerful approach for experimental modulation of sensory signaling and potentially for translation into therapy for chronic pain. This can be efficiently achieved by the use of recombinant adeno-associated virus (rAAV) in conjunction with nociceptor-specific regulatory transgene cassettes. Among different routes of delivery, direct injection into the dorsal root ganglia (DRGs) offers the most efficient AAV-mediated gene transfer selectively into the peripheral sensory nervous system. Here, we briefly discuss the advantages and applications of intraganglionic microinjection, and then provide a detailed approach for DRG injection, including a list of the necessary materials and description of a method for performing DRG microinjection experiments. We also discuss our experience with several adeno-associated virus (AAV) options for in vivo transgene expression in DRG neurons.
Author List
Yu H, Fischer G, Hogan QHAuthors
Quinn H. Hogan MD Professor in the Anesthesiology department at Medical College of WisconsinHongwei Yu MD Professor in the Anesthesiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsChronic Pain
Dependovirus
Ganglia, Spinal
Gene Transfer Techniques
Genetic Therapy
Genetic Vectors
Male
Microinjections
Rats
Rats, Sprague-Dawley
Transduction, Genetic
