Onchocerca volvulus recombinant antigen: physical characterization and clinical correlates with serum reactivity. J Infect Dis 1989 Sep;160(3):521-9
Date
09/01/1989Pubmed ID
2760503DOI
10.1093/infdis/160.3.521Scopus ID
2-s2.0-0024410195 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
An Onchocerca volvulus cDNA clone expressing epitopes found in adult and larval parasites, designated lambda RAL-2, was derived from a 1,000-base message present in adult O. volvulus, which encodes a protein with an apparent molecular weight of 17,000. This protein does not appear to be extensively posttranslationally modified. Serum samples from 52 individuals exposed to O. volvulus were examined for antibodies recognizing the lambda RAL-2 recombinant antigen; 77% produced such antibodies. In addition, individuals producing antibodies recognizing the recombinant antigen were significantly less likely to develop some aspects of ocular pathology associated with O. volvulus infection than were individuals who did not do so. These results suggest that recombinant antigens such as that produced by lambda RAL-2 may be useful in attempts to understand the mechanism of O. volvulus-induced ocular pathology.
Author List
Gallin MY, Tan M, Kron MA, Rechnitzer D, Greene BM, Newland HS, White AT, Taylor HR, Unnasch TRAuthor
Michael Kron MD Director, Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAmino Acid Sequence
Animals
Antigens, Helminth
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
DNA
Female
Humans
Immune Sera
Larva
Male
Molecular Sequence Data
Molecular Weight
Onchocerca
Onchocerciasis
Recombinant Fusion Proteins
Restriction Mapping
