Bacterial catalysis of nitrosation: involvement of the nar operon of Escherichia coli. J Bacteriol 1988 Jan;170(1):359-64
Date
01/01/1988Pubmed ID
3275620Pubmed Central ID
PMC210650DOI
10.1128/jb.170.1.359-364.1988Scopus ID
2-s2.0-0023742156 (requires institutional sign-in at Scopus site) 60 CitationsAbstract
We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions by nitrite and nitrate, that formate is the most efficient electron donor for this reaction, and that nitrosation may be catalyzed by nitrate reductase (EC 1.7.99.4). The narG mutants defective in nitrate reductase do not catalyze nitrosation, and the fnr gene is essential for nitrosation. Induction by nitrite or nitrate of nitrosation, N2O production, and nitrate reductase activity all require the narL gene.
Author List
Ralt D, Wishnok JS, Fitts R, Tannenbaum SRAuthor
Robert Fitts PhD Professor in the Biological Sciences department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
2-NaphthylamineAnaerobiosis
Catalysis
Chemical Phenomena
Chemistry
Escherichia coli
Formates
Genes, Bacterial
Morpholines
Mutation
Nitrate Reductases
Nitrates
Nitrites
Nitrosamines
Nitroso Compounds
Operon
