Expression deregulation of mir31 and CXCL12 in two types of oral precancers and cancer: importance in progression of precancer and cancer. Sci Rep 2016 Sep 06;6:32735
Date
09/07/2016Pubmed ID
27597234Pubmed Central ID
PMC5011738DOI
10.1038/srep32735Scopus ID
2-s2.0-84986296776 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
Oral cancer generally progresses from precancerous lesions such as leukoplakia (LK), lichen planus (LP) and oral submucous fibrosis (OSMF). Since few of these precancers progress to cancers; it is worth to identify biological molecules that may play important roles in progression. Here, expression deregulation of 7 miRNAs (mir204, mir31, mir31*, mir133a, mir7, mir206 and mir1293) and their possible target genes in 23 cancers, 18 LK, 12 LP, 23 OSMF tissues compared to 20 healthy tissues was determined by qPCR method. Expression of mir7, mir31, mir31* and mir1293 was upregulated and that of mir133a, mir204 and mir206 was downregulated in cancer. Expression of most of these miRNAs was also upregulated in LK and LP tissues but not in OSMF. Expression deregulation of some of the target genes was also determined in cancer, LK and LP tissues. Significant upregulation of mir31 and downregulation of its target gene, CXCL12, in cancer, LK and LP tissues suggest their importance in progression of precancer to cancer. Expression upregulation of mir31 was also validated using GEO data sets. Although sample size is low, novelty of this work lies in studying expression deregulation of miRNAs and target genes in oral cancer and three types of precancerous lesions.
Author List
Chattopadhyay E, Singh R, Ray A, Roy R, De Sarkar N, Paul RR, Pal M, Aich R, Roy BAuthor
Navonil De Sarkar PhD Assistant Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAged
Aged, 80 and over
Chemokine CXCL12
Disease Progression
Down-Regulation
Humans
Leukoplakia, Oral
MicroRNAs
Middle Aged
Mouth Mucosa
Mouth Neoplasms
Precancerous Conditions
Up-Regulation