Faculty Collaboration Database

An extracorporeal blood-cleansing device for sepsis therapy. Nat Med 2014 Oct;20(10):1211-6

Date

09/14/2014

Pubmed ID

25216635

DOI

10.1038/nm.3640

Scopus ID

2-s2.0-84911445227 (requires institutional sign-in at Scopus site)   245 Citations

Abstract

Here we describe a blood-cleansing device for sepsis therapy inspired by the spleen, which can continuously remove pathogens and toxins from blood without first identifying the infectious agent. Blood flowing from an infected individual is mixed with magnetic nanobeads coated with an engineered human opsonin--mannose-binding lectin (MBL)--that captures a broad range of pathogens and toxins without activating complement factors or coagulation. Magnets pull the opsonin-bound pathogens and toxins from the blood; the cleansed blood is then returned back to the individual. The biospleen efficiently removes multiple Gram-negative and Gram-positive bacteria, fungi and endotoxins from whole human blood flowing through a single biospleen unit at up to 1.25 liters per h in vitro. In rats infected with Staphylococcus aureus or Escherichia coli, the biospleen cleared >90% of bacteria from blood, reduced pathogen and immune cell infiltration in multiple organs and decreased inflammatory cytokine levels. In a model of endotoxemic shock, the biospleen increased survival rates after a 5-h treatment.

Author List

Kang JH, Super M, Yung CW, Cooper RM, Domansky K, Graveline AR, Mammoto T, Berthet JB, Tobin H, Cartwright MJ, Watters AL, Rottman M, Waterhouse A, Mammoto A, Gamini N, Rodas MJ, Kole A, Jiang A, Valentin TM, Diaz A, Takahashi K, Ingber DE

Authors

Akiko Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin
Tadanori Mammoto MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Artificial Organs
Biomedical Engineering
Biomimetic Materials
Endotoxins
Equipment Design
Escherichia coli
Extracorporeal Circulation
Humans
Magnetics
Male
Mannose-Binding Lectin
Microfluidic Analytical Techniques
Molecular Sequence Data
Opsonin Proteins
Rats
Rats, Wistar
Sepsis
Spleen
Staphylococcus aureus