Potential of Horse Apple Isoflavones in Targeting Inflammation and Tau Protein Fibrillization. Nat Prod Commun 2015 Sep;10(9):1577-80
Date
11/26/2015Pubmed ID
26594763Scopus ID
2-s2.0-84954624058 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
In our ongoing search for anti-inflammatory and neuroprotective agents of natural origin, the total methanolic extract (MPE) of horse apple (Maclura pomifera) and its two major prenylated isoflavones, osajin (OSA) and pomiferin (POM), were evaluated in vitro for their ability to affect four mediators of inflammation and to inhibit tau protein fibrillization. The two isoflavones were effective in enhancing the activity of NSAID activated gene (NAG-1) at 2.5 pg/mL (1.5-1.8 fold increase) and inhibiting iNOS and NF-κB activity with IC50 values in the range of 6-13 µg/mL. Pomiferin also inhibited intracellular oxidative stress with IC50 of 3.3 µg/mL, while osajin did not show any effect. The extract activated NAG-1 and inhibited iNOS and oxidative stress without affecting NF-κB. As observed by transmission electron microscopy (TEM), MPE, OSA and POM also inhibited arachidonic acid-induced tau fibrillization in a concentration-dependent manner.
Author List
Abourashed EA, Abraha A, Khan SI, McCants T, Awan SAuthor
Ehab A. Abourashed PhD Professor in the School of Pharmacy Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AntioxidantsCell Line, Tumor
Fruit
Gene Expression Regulation
Growth Differentiation Factor 15
Humans
Inflammation
Isoflavones
Maclura
Molecular Structure
Nitric Oxide Synthase Type II
tau Proteins
