Transport of harman alkaloids across Caco-2 cell monolayers. Chem Pharm Bull (Tokyo) 2004 Apr;52(4):394-7
Date
04/02/2004Pubmed ID
15056950DOI
10.1248/cpb.52.394Scopus ID
2-s2.0-7444267875 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
This study examined the intestinal transport of five harman alkaloids using the Caco-2 cell monolayer as a model of the human intestinal mucosa. Transport parameters, permeability coefficients and percent transports, were calculated and compared under identical conditions with atenolol. Permeability coefficients were also compared with the reported values for model compounds like mannitol, propranolol and glucose. Sodium fluorescein was used as the marker for paracellular leakage. These alkaloids, in the concentration range of 250-500 microM, demonstrated substantial transport across the monolayer with moderate to high efflux rates and permeability coefficients. The transport was linear with time and was concentration dependent.
Author List
Khan SI, Abourashed EA, Khan IA, Walker LAAuthor
Ehab A. Abourashed PhD Professor in the School of Pharmacy Administration department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adrenergic alpha-AntagonistsAlgorithms
Alkaloids
Atenolol
Biological Transport, Active
Caco-2 Cells
Chromatography, High Pressure Liquid
Fluoresceins
Fluorescent Dyes
Harmine
Humans