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Transport of harman alkaloids across Caco-2 cell monolayers. Chem Pharm Bull (Tokyo) 2004 Apr;52(4):394-7

Date

04/02/2004

Pubmed ID

15056950

DOI

10.1248/cpb.52.394

Scopus ID

2-s2.0-7444267875 (requires institutional sign-in at Scopus site)   24 Citations

Abstract

This study examined the intestinal transport of five harman alkaloids using the Caco-2 cell monolayer as a model of the human intestinal mucosa. Transport parameters, permeability coefficients and percent transports, were calculated and compared under identical conditions with atenolol. Permeability coefficients were also compared with the reported values for model compounds like mannitol, propranolol and glucose. Sodium fluorescein was used as the marker for paracellular leakage. These alkaloids, in the concentration range of 250-500 microM, demonstrated substantial transport across the monolayer with moderate to high efflux rates and permeability coefficients. The transport was linear with time and was concentration dependent.

Author List

Khan SI, Abourashed EA, Khan IA, Walker LA

Author

Ehab A. Abourashed PhD Professor in the School of Pharmacy Administration department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adrenergic alpha-Antagonists
Algorithms
Alkaloids
Atenolol
Biological Transport, Active
Caco-2 Cells
Chromatography, High Pressure Liquid
Fluoresceins
Fluorescent Dyes
Harmine
Humans