Longitudinal analysis of hepatic transcriptome and serum metabolome demonstrates altered lipid metabolism following the onset of hyperglycemia in spontaneously diabetic biobreeding rats. PLoS One 2017;12(2):e0171372
Date
02/14/2017Pubmed ID
28192442Pubmed Central ID
PMC5305198DOI
10.1371/journal.pone.0171372Scopus ID
2-s2.0-85012165803 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Type 1 diabetes is associated with abberations of fat metabolism before and after the clinical onset of disease. It has been hypothesized that the absence of the effect of insulin in the liver contributes to reduced hepatic fat synthesis. We measured hepatic gene expression and serum metabolites before and after the onset of hyperglycemia in a BioBreeding rat model of type 1 diabetes. Functional pathway annotation identified that lipid metabolism was differentially expressed in hyperglycemic rats and that these pathways significantly overlapped with genes regulated by insulin. 17 serum metabolites significantly changed in concentration. All but 2 of the identified metabolites had previously been reported in type 1 diabetes, and carbohydrates were overall the most upregulated class of metabolites. We conclude that lack of insulin in the liver contributes to the changes in fat metabolism observed in type 1 diabetes. Further studies are needed to understand the clinical consequences of a lack of insulin in the liver in patients with type 1 diabetes.
Author List
Regnell SE, Hessner MJ, Jia S, Åkesson L, Stenlund H, Moritz T, La Torre D, Lernmark ÅAuthor
Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Glucose
Breeding
Diabetes Mellitus, Type 1
Female
Gas Chromatography-Mass Spectrometry
Gene Expression Profiling
Hyperglycemia
Insulin
Lipid Metabolism
Liver
Male
Metabolome
Metabolomics
Rats, Inbred BB
Rats, Inbred F344
Signal Transduction
Time Factors
Transcriptome
