Spin-labeled lipid A. Anal Biochem 2008 Nov 15;382(2):129-31
Date
09/02/2008Pubmed ID
18760259Pubmed Central ID
PMC2570965DOI
10.1016/j.ab.2008.08.001Scopus ID
2-s2.0-52049104414 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Lipopolysaccharide (LPS), a major component of the outer membranes of gram-negative bacteria, is composed of a polysaccharide chain attached to a lipid A base that contains a disaccharide headgroup with two negative phosphate groups and at least four acyl chains. Lipid A is an essential component of the membranes of a large number of bacteria and is also a substrate for a wide variety of proteins. Here we report the synthesis of a nitroxide spin-labeled lipid A, characterize its localization at the membrane bilayer surface, and demonstrate that it remains a viable substrate for the Escherichia coli lipid flippase MsbA.
Author List
Bretscher LE, Buchaklian AH, Klug CSAuthor
Candice S. Klug PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Escherichia coliLipid A
Lipid Bilayers
Lipopolysaccharides
Spin Labels