Medical College of Wisconsin
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Spin-labeled lipid A. Anal Biochem 2008 Nov 15;382(2):129-31

Date

09/02/2008

Pubmed ID

18760259

Pubmed Central ID

PMC2570965

DOI

10.1016/j.ab.2008.08.001

Scopus ID

2-s2.0-52049104414 (requires institutional sign-in at Scopus site)   5 Citations

Abstract

Lipopolysaccharide (LPS), a major component of the outer membranes of gram-negative bacteria, is composed of a polysaccharide chain attached to a lipid A base that contains a disaccharide headgroup with two negative phosphate groups and at least four acyl chains. Lipid A is an essential component of the membranes of a large number of bacteria and is also a substrate for a wide variety of proteins. Here we report the synthesis of a nitroxide spin-labeled lipid A, characterize its localization at the membrane bilayer surface, and demonstrate that it remains a viable substrate for the Escherichia coli lipid flippase MsbA.

Author List

Bretscher LE, Buchaklian AH, Klug CS

Author

Candice S. Klug PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Escherichia coli
Lipid A
Lipid Bilayers
Lipopolysaccharides
Spin Labels