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Candice S. Klug PhD

Candice S. Klug PhD profile photo picture


Institution: Medical College of Wisconsin
Department: Biophysics
Program: Research

Member of the Cancer Center

Methodologies and Techniques

  • ATP-Binding Cassette Transporters
  • Adenosine Triphosphatases
  • Amino Acid Motifs
  • Arrestin
  • Arrestins
  • Bacterial Proteins
  • Electron Spin Resonance Spectroscopy
  • Escherichia coli Proteins
  • Lipid A
  • Lipopolysaccharides
  • Liposomes
  • Membrane Proteins
  • Membrane Transport Proteins
  • Protein Conformation
  • Protein Structure, Quaternary
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Spin Labels
  • MsbA
  • ArnT
  • LptA
  • site-directed spin labeling
  • electron paramagnetic resonance spectroscopy
  • LptC
  • Leadership Positions

  • Training Program Director, National Biomedical EPR Center, 2003-present
  • Chair, Graduate School Curriculum and Programs Committee, 2010-2013
  • Chair, Women's Faculty Council, 2006-2007
  • Acting Director, IDP Program in Biomedical Sciences, 2019-2020
  • Associate Director, National Biomedical EPR Center, 2014-2016
  • President, MCW Faculty Council, 2016-2017
  • Director, National Biomedical EPR Center, 2016 - present
  • Chair, Women in Science Advisory Committee, 2011 - 2016
  • Program Director, Biophysics Graduate Program, 2013 - present
  • MCW Program / Core Facilities

  • Interdisciplinary Program in the Biomedical Sciences
  • National Biomedical EPR Center
  • Biophysics Graduate Program
  • Publications (48)

  • A non-GPCR binding partner interacts with a novel surface on β-arrestin1 to mediate GPCR signaling. (Zhuo Y, Gurevich VV, Vishnivetskiy SA, Klug CS, Marchese A) J Biol Chem 2020 Aug 04 PMID: 32753481 08/06/2020    
  • Rutile dielectric loop-gap resonator for X-band EPR spectroscopy of small aqueous samples. (Mett RR, Sidabras JW, Anderson JR, Klug CS, Hyde JS) J Magn Reson 2019 10;307:106585 PMID: 31499469 PMCID: PMC6948142 SCOPUS ID: 2-s2.0-85071859156 09/10/2019       2 Citations
  • Disruption of the E. coli LptC dimerization interface and characterization of lipopolysaccharide and LptA binding to monomeric LptC. (Schultz KM, Fischer MA, Noey EL, Klug CS) Protein Sci 2018 08;27(8):1407-1417 PMID: 29672978 PMCID: PMC6153404 SCOPUS ID: 2-s2.0-85047394661 04/20/2018       1 Citation
  • High-pressure EPR spectroscopy studies of the E. coli lipopolysaccharide transport proteins LptA and LptC. (Schultz KM, Klug CS) Appl Magn Reson 2017 Dec;48(11-12):1341-1353 PMID: 29332998 PMCID: PMC5761346 01/16/2018    
  • Structural basis of arrestin-3 activation and signaling. (Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TM) Nat Commun 2017 11 10;8(1):1427 PMID: 29127291 PMCID: PMC5681653 SCOPUS ID: 2-s2.0-85033606940 11/12/2017       34 Citations
  • Characterization of and lipopolysaccharide binding to the E. coli LptC protein dimer. (Schultz KM, Klug CS) Protein Sci 2018 02;27(2):381-389 PMID: 29024084 PMCID: PMC5775163 SCOPUS ID: 2-s2.0-85040841909 10/13/2017       4 Citations
  • Directed evolution provides insight into conformational substrate sampling by SrtA. (Suliman M, Santosh V, Seegar TCM, Dalton AC, Schultz KM, Klug CS, Barton WA) PLoS One 2017;12(8):e0184271 PMID: 28859178 PMCID: PMC5578623 SCOPUS ID: 2-s2.0-85028825732 09/01/2017       4 Citations
  • Overview of arrestin mediated signaling with receptors and non-receptor binding partners (Zhuo Y, Klug CS) The Structural Basis of Arrestin Functions 24 May 2017:19-29 SCOPUS ID: 2-s2.0-85034009393 05/24/2017    
  • GPCR footprint on arrestins and manipulation of receptor specificity (Vishnivetskiy SA, Hubbell WL, Klug CS, Gurevich VV) The Structural Basis of Arrestin Functions 24 May 2017:133-140 SCOPUS ID: 2-s2.0-85033979110 05/24/2017    
  • Active conformations of arrestins: Expected and unexpected changes (Bandyopadhyay A, Zhuo Y, Hubbell WL, Klug CS, Gurevich VV, Ernst OP) The Structural Basis of Arrestin Functions 24 May 2017:159-173 SCOPUS ID: 2-s2.0-85034008570 05/24/2017    
  • Lipopolysaccharide binding to the periplasmic protein LptA. (Schultz KM, Lundquist TJ, Klug CS) Protein Sci 2017 Aug;26(8):1517-1523 PMID: 28419595 PMCID: PMC5521551 SCOPUS ID: 2-s2.0-85018394516 04/19/2017       12 Citations
  • Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation. (Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Belcher Dufrisne M, Kloss B, Kloppmann E, Rost B, Klug CS, Trent MS, Shapiro L, Mancia F) Science 2016 Feb 05;351(6273):608-12 PMID: 26912703 PMCID: PMC4963604 SCOPUS ID: 2-s2.0-84957545547 02/26/2016       48 Citations
  • Full engagement of liganded maltose-binding protein stabilizes a semi-open ATP-binding cassette dimer in the maltose transporter. (Alvarez FJ, Orelle C, Huang Y, Bajaj R, Everly RM, Klug CS, Davidson AL) Mol Microbiol 2015 Dec;98(5):878-94 PMID: 26268698 PMCID: PMC4715599 SCOPUS ID: 2-s2.0-84983126744 08/14/2015       19 Citations
  • Identification of receptor binding-induced conformational changes in non-visual arrestins. (Zhuo Y, Vishnivetskiy SA, Zhan X, Gurevich VV, Klug CS) J Biol Chem 2014 Jul 25;289(30):20991-1002 PMID: 24867953 PMCID: PMC4110305 SCOPUS ID: 2-s2.0-84905370362 05/29/2014       32 Citations
  • Self-association of arrestin family members. (Chen Q, Zhuo Y, Kim M, Hanson SM, Francis DJ, Vishnivetskiy SA, Altenbach C, Klug CS, Hubbell WL, Gurevich VV) Handb Exp Pharmacol 2014;219:205-23 PMID: 24292832 PMCID: PMC4512752 SCOPUS ID: 2-s2.0-84958539644 12/03/2013       15 Citations
  • Site-directed spin labeling reveals pentameric ligand-gated ion channel gating motions. (Dellisanti CD, Ghosh B, Hanson SM, Raspanti JM, Grant VA, Diarra GM, Schuh AM, Satyshur K, Klug CS, Czajkowski C) PLoS Biol 2013 Nov;11(11):e1001714 PMID: 24260024 PMCID: PMC3833874 SCOPUS ID: 2-s2.0-84889038866 11/22/2013       33 Citations
  • Disruption of LptA oligomerization and affinity of the LptA-LptC interaction. (Schultz KM, Feix JB, Klug CS) Protein Sci 2013 Nov;22(11):1639-45 PMID: 24123237 PMCID: PMC3831678 SCOPUS ID: 2-s2.0-84892738005 10/15/2013       18 Citations
  • EPR spectroscopy of MolB2C2-a reveals mechanism of transport for a bacterial type II molybdate importer. (Rice AJ, Alvarez FJ, Schultz KM, Klug CS, Davidson AL, Pinkett HW) J Biol Chem 2013 Jul 19;288(29):21228-35 PMID: 23709218 PMCID: PMC3774390 SCOPUS ID: 2-s2.0-84880536145 05/28/2013       19 Citations
  • Probing Protein Secondary Structure using EPR: Investigating a Dynamic Region of Visual Arrestin. (Francis DJ, Hubbell WL, Klug CS) Appl Magn Reson 2012 Oct;43(3):405-419 PMID: 25419051 PMCID: PMC4240029 10/01/2012    
  • Concentration-dependent oligomerization and oligomeric arrangement of LptA. (Merten JA, Schultz KM, Klug CS) Protein Sci 2012 Feb;21(2):211-8 PMID: 22109962 PMCID: PMC3324765 SCOPUS ID: 2-s2.0-84856173418 11/24/2011       41 Citations
  • Few residues within an extensive binding interface drive receptor interaction and determine the specificity of arrestin proteins. (Vishnivetskiy SA, Gimenez LE, Francis DJ, Hanson SM, Hubbell WL, Klug CS, Gurevich VV) J Biol Chem 2011 Jul 08;286(27):24288-99 PMID: 21471193 PMCID: PMC3129209 SCOPUS ID: 2-s2.0-79959862379 04/08/2011       86 Citations
  • Characterization of the E506Q and H537A dysfunctional mutants in the E. coli ABC transporter MsbA. (Schultz KM, Merten JA, Klug CS) Biochemistry 2011 May 10;50(18):3599-608 PMID: 21462989 PMCID: PMC3128438 SCOPUS ID: 2-s2.0-79955628177 04/06/2011       20 Citations
  • Effects of the L511P and D512G mutations on the Escherichia coli ABC transporter MsbA. (Schultz KM, Merten JA, Klug CS) Biochemistry 2011 Apr 05;50(13):2594-602 PMID: 21344946 PMCID: PMC3110719 SCOPUS ID: 2-s2.0-79953175250 02/25/2011       15 Citations
  • Identification of a functionally important loop in Salmonella typhimurium ArnT. (Impellitteri NA, Merten JA, Bretscher LE, Klug CS) Biochemistry 2010 Jan 12;49(1):29-35 PMID: 19947657 PMCID: PMC2805049 SCOPUS ID: 2-s2.0-73449121971 12/02/2009       8 Citations
  • The role of arrestin alpha-helix I in receptor binding. (Vishnivetskiy SA, Francis D, Van Eps N, Kim M, Hanson SM, Klug CS, Hubbell WL, Gurevich VV) J Mol Biol 2010 Jan 08;395(1):42-54 PMID: 19883657 PMCID: PMC2787876 SCOPUS ID: 2-s2.0-70450225035 11/04/2009       49 Citations
  • Functionally important ATP binding and hydrolysis sites in Escherichia coli MsbA. (Westfahl KM, Merten JA, Buchaklian AH, Klug CS) Biochemistry 2008 Dec 30;47(52):13878-86 PMID: 19053284 PMCID: PMC2637178 SCOPUS ID: 2-s2.0-58849132410 12/05/2008       24 Citations
  • HLA-DM mediates epitope selection by a "compare-exchange" mechanism when a potential peptide pool is available. (Ferrante A, Anderson MW, Klug CS, Gorski J) PLoS One 2008;3(11):e3722 PMID: 19005572 PMCID: PMC2580030 SCOPUS ID: 2-s2.0-56649109618 11/14/2008       24 Citations
  • Spin-labeled lipid A. (Bretscher LE, Buchaklian AH, Klug CS) Anal Biochem 2008 Nov 15;382(2):129-31 PMID: 18760259 PMCID: PMC2570965 SCOPUS ID: 2-s2.0-52049104414 09/02/2008       4 Citations
  • Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter. (Orelle C, Ayvaz T, Everly RM, Klug CS, Davidson AL) Proc Natl Acad Sci U S A 2008 Sep 02;105(35):12837-42 PMID: 18725638 PMCID: PMC2529024 SCOPUS ID: 2-s2.0-51349104365 08/30/2008       86 Citations
  • A model for the solution structure of the rod arrestin tetramer. (Hanson SM, Dawson ES, Francis DJ, Van Eps N, Klug CS, Hubbell WL, Meiler J, Gurevich VV) Structure 2008 Jun;16(6):924-34 PMID: 18547524 PMCID: PMC2464289 SCOPUS ID: 2-s2.0-44649193852 06/13/2008       54 Citations
  • Methods and applications of site-directed spin labeling EPR spectroscopy. (Klug CS, Feix JB) Methods Cell Biol 2008;84:617-58 PMID: 17964945 SCOPUS ID: 2-s2.0-35448961949 10/30/2007       119 Citations
  • Arrestin mobilizes signaling proteins to the cytoskeleton and redirects their activity. (Hanson SM, Cleghorn WM, Francis DJ, Vishnivetskiy SA, Raman D, Song X, Nair KS, Slepak VZ, Klug CS, Gurevich VV) J Mol Biol 2007 Apr 27;368(2):375-87 PMID: 17359998 PMCID: PMC1904837 SCOPUS ID: 2-s2.0-33947607229 03/16/2007       88 Citations
  • Structure and function of the visual arrestin oligomer. (Hanson SM, Van Eps N, Francis DJ, Altenbach C, Vishnivetskiy SA, Arshavsky VY, Klug CS, Hubbell WL, Gurevich VV) EMBO J 2007 Mar 21;26(6):1726-36 PMID: 17332750 PMCID: PMC1829381 SCOPUS ID: 2-s2.0-33947577189 03/03/2007       88 Citations
  • Arrestin binding to calmodulin: a direct interaction between two ubiquitous signaling proteins. (Wu N, Hanson SM, Francis DJ, Vishnivetskiy SA, Thibonnier M, Klug CS, Shoham M, Gurevich VV) J Mol Biol 2006 Dec 15;364(5):955-63 PMID: 17054984 PMCID: PMC1783800 SCOPUS ID: 2-s2.0-33751079911 10/24/2006       62 Citations
  • Characterization of the LSGGQ and H motifs from the Escherichia coli lipid A transporter MsbA. (Buchaklian AH, Klug CS) Biochemistry 2006 Oct 17;45(41):12539-46 PMID: 17029409 PMCID: PMC2526060 SCOPUS ID: 2-s2.0-33750038243 10/13/2006       26 Citations
  • Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. (Hanson SM, Francis DJ, Vishnivetskiy SA, Kolobova EA, Hubbell WL, Klug CS, Gurevich VV) Proc Natl Acad Sci U S A 2006 Mar 28;103(13):4900-5 PMID: 16547131 PMCID: PMC1458767 SCOPUS ID: 2-s2.0-33645506641 03/21/2006       144 Citations
  • Visual arrestin binding to microtubules involves a distinct conformational change. (Hanson SM, Francis DJ, Vishnivetskiy SA, Klug CS, Gurevich VV) J Biol Chem 2006 Apr 07;281(14):9765-72 PMID: 16461350 PMCID: PMC2430877 SCOPUS ID: 2-s2.0-33646942808 02/08/2006       70 Citations
  • Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium. (Bretscher LE, Morrell MT, Funk AL, Klug CS) Protein Expr Purif 2006 Mar;46(1):33-9 PMID: 16226890 SCOPUS ID: 2-s2.0-32644459308 10/18/2005       11 Citations
  • Concentration by centrifugation for gas exchange EPR oximetry measurements with loop-gap resonators. (Subczynski WK, Felix CC, Klug CS, Hyde JS) J Magn Reson 2005 Oct;176(2):244-8 PMID: 16040261 SCOPUS ID: 2-s2.0-25144497630 07/26/2005       31 Citations
  • Characterization of the Walker A motif of MsbA using site-directed spin labeling electron paramagnetic resonance spectroscopy. (Buchaklian AH, Klug CS) Biochemistry 2005 Apr 12;44(14):5503-9 PMID: 15807544 SCOPUS ID: 2-s2.0-16844381206 04/06/2005       41 Citations
  • Multiquantum EPR spectroscopy of spin-labeled arrestin K267C at 35 GHz. (Klug CS, Camenisch TG, Hubbell WL, Hyde JS) Biophys J 2005 May;88(5):3641-7 PMID: 15749769 PMCID: PMC1305511 SCOPUS ID: 2-s2.0-17844402249 03/08/2005       18 Citations
  • Resting state conformation of the MsbA homodimer as studied by site-directed spin labeling. (Buchaklian AH, Funk AL, Klug CS) Biochemistry 2004 Jul 06;43(26):8600-6 PMID: 15222771 SCOPUS ID: 2-s2.0-3142580842 06/30/2004       33 Citations
  • Maltose-binding protein is open in the catalytic transition state for ATP hydrolysis during maltose transport. (Austermuhle MI, Hall JA, Klug CS, Davidson AL) J Biol Chem 2004 Jul 02;279(27):28243-50 PMID: 15117946 SCOPUS ID: 2-s2.0-3142619074 05/01/2004       60 Citations
  • Calcium-sensitive regions of GCAP1 as observed by chemical modifications, fluorescence, and EPR spectroscopies. (Sokal I, Li N, Klug CS, Filipek S, Hubbell WL, Baehr W, Palczewski K) J Biol Chem 2001 Nov 16;276(46):43361-73 PMID: 11524415 PMCID: PMC1363678 SCOPUS ID: 2-s2.0-0035900764 08/29/2001       35 Citations
  • Guanidine hydrochloride unfolding of a transmembrane beta-strand in FepA using site-directed spin labeling. (Klug CS, Feix JB) Protein Sci 1998 Jun;7(6):1469-76 PMID: 9655352 PMCID: PMC2144043 SCOPUS ID: 2-s2.0-0031777549 07/09/1998       30 Citations
  • Ligand-induced conformational change in the ferric enterobactin receptor FepA as studied by site-directed spin labeling and time-domain ESR. (Klug CS, Eaton SS, Eaton GR, Feix JB) Biochemistry 1998 Jun 23;37(25):9016-23 PMID: 9636045 SCOPUS ID: 2-s2.0-0032560604 06/24/1998       24 Citations
  • Mapping of the residues involved in a proposed beta-strand located in the ferric enterobactin receptor FepA using site-directed spin-labeling. (Klug CS, Su W, Feix JB) Biochemistry 1997 Oct 21;36(42):13027-33 PMID: 9335564 SCOPUS ID: 2-s2.0-0030693909 10/23/1997       51 Citations
  • Denaturant unfolding of the ferric enterobactin receptor and ligand-induced stabilization studied by site-directed spin labeling. (Klug CS, Su W, Liu J, Klebba PE, Feix JB) Biochemistry 1995 Oct 31;34(43):14230-6 PMID: 7578022 SCOPUS ID: 2-s2.0-0028858144 10/31/1995       37 Citations
  • Last update: 08/06/2020
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