Faculty Collaboration Database

Effect of Angiotensin II and ACTH on Adrenal Blood Flow in the Male Rat Adrenal Gland In Vivo. Endocrinology 2018 Jan 01;159(1):217-226

Date

11/16/2017

Pubmed ID

29140411

Pubmed Central ID

PMC5761607

DOI

10.1210/en.2016-1594

Scopus ID

2-s2.0-85040721869 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Angiotensin II (Ang II) and adrenocorticotropic hormone (ACTH) regulate adrenal vascular tone in vitro through endothelial and zona glomerulosa cell-derived mediators. The role of these mediators in regulating adrenal blood flow (ABF) and mean arterial pressure (MAP) was examined in anesthetized rats. Ang II (0.01 to 100 ng/kg) increased ABF [maximal increase of 97.2 ± 6.9 perfusion units (PUs) at 100 ng/kg] and MAP (basal, 115 ± 7 mm Hg; Ang II, 163 ± 5 mm Hg). ACTH (0.1 to 1000 ng/kg) also increased ABF (maximum increase of 91.4 ± 10.7 PU) without changing MAP. ABF increase by Ang II was partially inhibited by the nitric oxide (NO) synthase inhibitor N-nitro-l-arginine methyl ester (L-NAME) (maximum increase of 72.9 ± 4.2 PU), the cytochrome P450 inhibitor miconazole (maximum increase of 39.1 ± 6.8 PU) and the epoxyeicosatrienoic acid (EET) antagonist 14,15-epoxyeicosa-5(Z)-enoic acid (14,15-EEZE) (maximum increase of 56.0 ± 13.7 PU) alone, whereas combined administration of miconazole and L-NAME (maximum increase of 16.40 ± 8.98 PU) ablated it. These treatments had no effect on MAP. Indomethacin did not affect the increase in ABF or MAP induced by Ang II. The ABF increase by ACTH was partially ablated by miconazole and 14,15-EEZE but not by L-NAME. Steroidogenic stimuli such as Ang II and ACTH increase ABF to promote oxygen and cholesterol delivery for steroidogenesis and aldosterone transport to its target tissues. The increases in ABF induced by Ang II are mediated by release of NO and EETs, whereas ABF increases with ACTH are mediated by EETs only.

Author List

Shah AJ, Kriska T, Gauthier KM, Falck JR, Campbell WB

Authors

William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
Tamas Kriska PhD Research Scientist I in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

8,11,14-Eicosatrienoic Acid
Adrenal Glands
Adrenocorticotropic Hormone
Angiotensin II
Animals
Cyclooxygenase Inhibitors
Cytochrome P-450 Enzyme Inhibitors
Eicosanoids
Endothelium, Vascular
Enzyme Inhibitors
Indomethacin
Injections, Intravenous
Male
Miconazole
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 2
Receptors, Corticotropin
Regional Blood Flow
Signal Transduction