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Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4. Methods Mol Biol 2018;1722:151-164

Date

12/22/2017

Pubmed ID

29264804

Pubmed Central ID

PMC6345166

DOI

10.1007/978-1-4939-7553-2_10

Scopus ID

2-s2.0-85038862440   6 Citations

Abstract

G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling.

Author List

Nevins AM, Marchese A

Author

Adriano Marchese PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Absorption, Physicochemical
Antibodies, Monoclonal
Cell Membrane
Chemokine CXCL12
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Dyes
HEK293 Cells
HeLa Cells
Humans
Ligands
Receptors, CXCR4