Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4. Methods Mol Biol 2018;1722:151-164
Date
12/22/2017Pubmed ID
29264804Pubmed Central ID
PMC6345166DOI
10.1007/978-1-4939-7553-2_10Scopus ID
2-s2.0-85038862440 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
G protein-coupled receptors (GPCRs) are cell surface receptors that relay extracellular signals to the inside of the cells. C-X-C chemokine receptor 4 (CXCR4) is a GPCR that undergoes receptor internalization and recycling upon stimulation with its cognate ligand, C-X-C chemokine 12 (CXCL12). Using this receptor/ligand pair we describe the use of two techniques, enzyme-linked immunosorbent assay (ELISA) and flow cytometry, widely used to quantify GPCR internalization from the plasma membrane and its return to the cell surface by recycling.
Author List
Nevins AM, Marchese AAuthor
Adriano Marchese PhD Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Absorption, PhysicochemicalAntibodies, Monoclonal
Cell Membrane
Chemokine CXCL12
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Dyes
HEK293 Cells
HeLa Cells
Humans
Ligands
Receptors, CXCR4