Faculty Collaboration Database

Feedback between tissue packing and neurogenesis in the zebrafish neural tube. Development 2018 May 04;145(9)

Date

04/22/2018

Pubmed ID

29678815

Pubmed Central ID

PMC5992593

DOI

10.1242/dev.157040

Scopus ID

2-s2.0-85046850961 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

Balancing the rate of differentiation and proliferation in developing tissues is essential to produce organs of robust size and composition. Although many molecular regulators have been established, how these connect to physical and geometrical aspects of tissue architecture is poorly understood. Here, using high-resolution timelapse imaging, we find that changes to cell geometry associated with dense tissue packing play a significant role in regulating differentiation rate in the zebrafish neural tube. Specifically, progenitors that are displaced away from the apical surface due to crowding, tend to differentiate in a Notch-dependent manner. Using simulations we show that interplay between progenitor density, cell shape and changes in differentiation rate could naturally result in negative-feedback control on progenitor cell number. Given these results, we suggest a model whereby differentiation rate is regulated by density dependent effects on cell geometry to: (1) correct variability in cell number; and (2) balance the rates of proliferation and differentiation over development to 'fill' the available space.

Author List

Hiscock TW, Miesfeld JB, Mosaliganti KR, Link BA, Megason SG

Authors

Brian A. Link PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
Joel Bryan Miesfeld PhD Assistant Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Differentiation
Cell Proliferation
Neural Stem Cells
Neural Tube
Neurogenesis
Receptors, Notch
Zebrafish
Zebrafish Proteins