Bone Marrow-derived CD8+ T Cells From Pediatric Leukemia Patients Express PD1 and Expand Ex Vivo Following Induction Chemotherapy. J Pediatr Hematol Oncol 2019 Nov;41(8):648-652
Date
06/19/2018Pubmed ID
29912035Pubmed Central ID
PMC6855330DOI
10.1097/MPH.0000000000001244Scopus ID
2-s2.0-85048631854 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Adoptive cell therapy (ACT) of chimeric antigen receptor T cells has demonstrated remarkable success for the treatment of pediatric B-cell leukemia. For patients who are not candidates for chimeric antigen receptor T-cell therapy, ACT using tumor antigen-experienced polyclonal T cells may be a treatment option. Since leukemic blasts reside in the bone marrow and bone marrow is a preferred site for homeostatic proliferation of cytotoxic memory CD8 T cells, we hypothesized that bone marrow would be a source of activated T cells. The aim of this study was to determine the feasibility of using bone marrow-derived T cells following postinduction chemotherapy for use in adoptive cell transfer. Matched patient samples of bone marrow and peripheral blood-derived T cells expanded ex vivo and displayed similar apoptotic profiles. Before activation and expansion, there was a significant increase in the percentage of bone marrow-derived CD8 T cells expressing activation markers PD1, CD45RO, and CD69 as compared with peripheral blood CD8 T cells. Considering, melanoma-reactive CD8 T cells reside in the subset of PD1CD8 T cells, the bone marrow may be an enriched source leukemic-specific T cells that can be used for ACT.
Author List
Palen K, Thakar M, Johnson BD, Gershan JAAuthor
Bryon D. Johnson PhD Adjunct Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antigens, DifferentiationApoptosis
Bone Marrow Cells
CD8-Positive T-Lymphocytes
Child
Female
Gene Expression Regulation, Leukemic
Humans
Immunologic Memory
Induction Chemotherapy
Leukemia
Male
Neoplasm Proteins
Programmed Cell Death 1 Receptor