Faculty Collaboration Database

Periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid. Mol Oral Microbiol 2012 Feb;27(1):45-56

Date

01/11/2012

Pubmed ID

22230465

Pubmed Central ID

PMC3257822

DOI

10.1111/j.2041-1014.2011.00631.x

Scopus ID

2-s2.0-84855753846 (requires institutional sign-in at Scopus site)   32 Citations

Abstract

In periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (NE) and protease 3 (PR3), the extracellular activity of which is tightly controlled by endogenous inhibitors. In vitro these inhibitors are degraded by gingipains, cysteine proteases produced by Porphyromonas gingivalis. The purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (GCF) in relation to periodontal infection. The GCF collected from 31 subjects (nine healthy controls, seven with gingivitis, five with aggressive periodontitis and 10 with chronic periodontitis) was analyzed for the levels of elafin and secretory leukocyte protease inhibitor (SLPI), two main tissue-derived inhibitors of neutrophil serine proteases. In parallel, activity of NE, PR3 and arginine-specific gingipains (Rgps) in GCF was measured. Finally loads of P. gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia and Treponema denticola were determined. The highest values of elafin were found in aggressive periodontitis and the lowest in controls. The quantity of elafin correlated positively with the load of P. gingivalis, Ta. forsythia and Tr. denticola, as well as with Rgps activity. In addition, NE activity was positively associated with the counts of those bacterial species, but not with the amount of elafin. In contrast, the highest concentrations of SLPI were found in periodontally healthy subjects whereas amounts of this inhibitor were significantly decreased in patients infected with P. gingivalis. Periodontopathogenic bacteria stimulate the release of NE and PR3, which activities escape the control through degradation of locally produced inhibitors (SLPI and elafin) by host-derived and bacteria-derived proteases.

Author List

Laugisch O, Schacht M, Guentsch A, Kantyka T, Sroka A, Stennicke HR, Pfister W, Sculean A, Potempa J, Eick S

Author

Arndt Geuntsch in the CTSI department at Medical College of Wisconsin - CTSI

MESH terms used to index this publication - Major topics in bold

Adhesins, Bacterial
Adult
Aggregatibacter actinomycetemcomitans
Aggressive Periodontitis
Bacteroides
Case-Control Studies
Chronic Periodontitis
Cysteine Endopeptidases
Elafin
Female
Gingival Crevicular Fluid
Gingivitis
Humans
Male
Middle Aged
Porphyromonas gingivalis
Proteinase Inhibitory Proteins, Secretory
Secretory Leukocyte Peptidase Inhibitor
Serine Proteases
Serine Proteinase Inhibitors
Statistics, Nonparametric
Treponema denticola