Faculty Collaboration Database

Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells 2009 Apr;27(4):806-11

Date

04/08/2009

Pubmed ID

19350680

Pubmed Central ID

PMC2895909

DOI

10.1002/stem.31

Scopus ID

2-s2.0-65649115029 (requires institutional sign-in at Scopus site)   327 Citations

Abstract

The potential for directed differentiation of human-induced pluripotent stem (iPS) cells to functional postmitotic neuronal phenotypes is unknown. Following methods shown to be effective at generating motor neurons from human embryonic stem cells (hESCs), we found that once specified to a neural lineage, human iPS cells could be differentiated to form motor neurons with a similar efficiency as hESCs. Human iPS-derived cells appeared to follow a normal developmental progression associated with motor neuron formation and possessed prototypical electrophysiological properties. This is the first demonstration that human iPS-derived cells are able to generate electrically active motor neurons. These findings demonstrate the feasibility of using iPS-derived motor neuron progenitors and motor neurons in regenerative medicine applications and in vitro modeling of motor neuron diseases.

Author List

Karumbayaram S, Novitch BG, Patterson M, Umbach JA, Richter L, Lindgren A, Conway AE, Clark AT, Goldman SA, Plath K, Wiedau-Pazos M, Kornblum HI, Lowry WE

Author

Michaela Patterson PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Cell Culture Techniques
Cell Differentiation
Cell Line
Cell Lineage
Embryonic Stem Cells
Humans
Motor Neuron Disease
Motor Neurons
Patch-Clamp Techniques
Pluripotent Stem Cells
Regenerative Medicine