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Phospholipase C gamma 2 is essential for specific functions of Fc epsilon R and Fc gamma R. J Immunol 2002 Dec 15;169(12):6743-52



Pubmed ID




Scopus ID

2-s2.0-0037114147   54 Citations


Phospholipase Cgamma2 (PLCgamma2) plays a critical role in the functions of the B cell receptor in B cells and of the FcRgamma chain-containing collagen receptor in platelets. Here we report that PLCgamma2 is also expressed in mast cells and monocytes/macrophages and is activated by cross-linking of Fc(epsilon)R and Fc(gamma)R. Although PLCgamma2-deficient mice have normal development and numbers of mast cells and monocytes/macrophages, we demonstrate that PLCgamma2 is essential for specific functions of Fc(epsilon)R and Fc(gamma)R. While PLCgamma2-deficient mast cells have normal mitogen-activated protein kinase activation and cytokine production at mRNA levels, the mutant cells have impaired Fc(epsilon)R-mediated Ca(2+) flux and inositol 1,4,5-trisphosphate production, degranulation, and cytokine secretion. As a physiological consequence of the effect of PLCgamma2 deficiency, the mutant mice are resistant to IgE-mediated cutaneous inflammatory skin reaction. Macrophages from PLCgamma2-deficient mice have no detectable Fc(gamma)R-mediated Ca(2+) flux; however, the mutant cells have normal Fc(gamma)R-mediated phagocytosis. Moreover, PLCgamma2 plays a nonredundant role in Fc(gamma)R-mediated inflammatory skin reaction.

Author List

Wen R, Jou ST, Chen Y, Hoffmeyer A, Wang D


Demin Wang PhD Assistant Professor in the Microbiology and Immunology department at Medical College of Wisconsin
Renren Wen PhD Associate Investigator in the Blood Research Institute department at BloodCenter of Wisconsin

MESH terms used to index this publication - Major topics in bold

Biological Transport
Cations, Divalent
Cell Degranulation
Enzyme Activation
Immunity, Innate
Immunoglobulin E
Macrophages, Peritoneal
Mast Cells
Mice, Knockout
Mitogen-Activated Protein Kinases
Passive Cutaneous Anaphylaxis
Phospholipase C gamma
Receptors, IgE
Receptors, IgG
Transcription, Genetic
Type C Phospholipases
jenkins-FCD Prod-469 c3fc8ab87196149f9b23743c01b947d47e7319e5