Persistence of Schistosoma japonicum DNA in a Kidney-Liver Transplant Recipient. Am J Trop Med Hyg 2019 Mar;100(3):584-587
Date
01/11/2019Pubmed ID
30628570Pubmed Central ID
PMC6402933DOI
10.4269/ajtmh.18-0752Scopus ID
2-s2.0-85062624381 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Mitochondrial genome analysis of Schistosoma japonicum suggests that diversity of intermediate host snails drove intra-species divergence during its expansion in Asia. We applied the knowledge of this genomic variation to study an unusual patient we recently diagnosed with schistosomiasis. The patient had not visited any schistosomiasis-endemic countries for more than 35 years and had no idea where she became infected. Unusual clinical features of this patient included the absence of egg granulomas in tissue and persistent noncalcified eggs despite multiple praziquantel (PZQ) treatments over 7 years. A digital droplet polymerase chair reaction (PCR) assay that specifically targets the schistosome 1,4 dihydronicotinamide adenine dinucleotide-1 (NADH1) dehydrogenase-1 mitochondrial gene successfully amplified parasite DNA extracted from colon biopsies. DNA sequence analysis of parasite DNA revealed that it was a Philippine strain of S. japonicum. Future molecular studies using stored DNA from patients such as this may provide new insight into why some persons do not respond well to PZQ treatment.
Author List
Kron M, Gordon C, Bauers T, Lu Z, Mahatme S, Shah J, Saeian K, McManus DPAuthors
Michael Kron MD Director, Professor in the Medicine department at Medical College of WisconsinSheran Mahatme DO Associate Professor in the Medicine department at Medical College of Wisconsin
Kia Saeian MD Professor in the Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AdultAnimals
Anthelmintics
Colon
DNA, Helminth
Humans
Kidney Transplantation
Liver Transplantation
Ovum
Phylogeny
Praziquantel
Schistosoma japonicum
Transplant Recipients
