Faculty Collaboration Database

Tissue and cell specific expression of a renin promoter-reporter gene construct in transgenic mice. Biochem Biophys Res Commun 1990 Jul 16;170(1):344-50

Date

07/16/1990

Pubmed ID

1695507

DOI

10.1016/0006-291x(90)91280-6

Scopus ID

2-s2.0-0025108262 (requires institutional sign-in at Scopus site)   70 Citations

Abstract

The tissue specific expression of a fusion transgene consisting of 4.6 kb of Ren-2 5' flanking sequence and the SV40 T antigen viral oncogene was analyzed by northern hybridization and immunocytochemistry. Among the 10 tissues examined, endogenous renin transcripts were identified in and restricted to kidney, submandibular gland, testes and ovary consistent with the expression pattern of the Ren-1c gene. In addition to these tissues, significant levels of transgene mRNA were detectable in the brain. Expression of the transgene was restricted to juxtaglomerular cells in the kidney and the granular convoluted tubule cells of submandibular gland. These results suggest that 4.6 Kb of Ren-2 5' flanking sequence is sufficient to confer tissue and cell specific expression upon an exogenous reporter gene.

Author List

Sigmund CD, Jones CA, Fabian JR, Mullins JJ, Gross KW

Author

Curt Sigmund PhD Chair, Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Female
Male
Mice
Mice, Transgenic
Organ Specificity
Promoter Regions, Genetic
RNA
Recombinant Fusion Proteins
Renin