Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Elevated mutant frequencies in lymphoid tissues persist throughout plasmacytoma development in BALB/c.lambdaLIZ mice. Cancer Res 1999 Aug 01;59(15):3621-6

Date

08/14/1999

Pubmed ID

10446972

Scopus ID

2-s2.0-0033179682   10 Citations

Abstract

Using the phage lambdaLIZ-based transgenic in vivo mutagenesis assay, the mean mutant frequencies in the target gene, lacI, were found to be significantly increased in lymphoid tissues of congenic BALB/c.lambdaLIZ N5 mice in the terminal stage of a plasmacytoma induction experiment, 213-280 days after the first i.p. injection of the plasmacytomagenic agent pristane (2,6,10,14-tetramethylpentadecane). In plasmacytoma-bearing mice (n = 7), mutant frequencies in the spleens and mesenteric lymph nodes were elevated 2.46-fold and 5.35-fold, respectively, when compared with age-matched controls. In plasmacytoma-negative mice (n = 11), mutant frequencies were increased 2.30-fold (spleens) and 3.48-fold (mesenteric nodes). These results, interpreted in conjunction with our previous findings (K. Felix et al., Cancer Res., 58: 1616-1619, 1998) of approximately 3-fold elevations in pristane-induced splenic mutagenesis on day 42 postpristane, indicate that increased mutant levels in lymphoid tissues persist throughout plasmacytomagenesis in genetically susceptible BALB/c mice.

Author List

Felix K, Kelliher KA, Bornkamm GW, Janz S

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Proteins
Carcinogens
DNA
DNA Mutational Analysis
DNA, Neoplasm
Disease Progression
Escherichia coli Proteins
Female
Genes, Reporter
Lac Operon
Lac Repressors
Lymph Nodes
Lymphoid Tissue
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Mutagenesis
Peritoneal Neoplasms
Plasmacytoma
Repressor Proteins
Spleen
Terpenes