Medical College of Wisconsin
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Articular cartilage vesicles generate calcium pyrophosphate dihydrate-like crystals in vitro. Arthritis Rheum 1992 Feb;35(2):231-40

Date

02/01/1992

Pubmed ID

1734912

DOI

10.1002/art.1780350218

Scopus ID

2-s2.0-0026532851 (requires institutional sign-in at Scopus site)   92 Citations

Abstract

OBJECTIVE: To identify the morphology of a mineral-forming of adult porcine hyaline articular cartilage digest and characterize the mineral it forms.

METHODS: Electron microscopy, Fourier transform infrared (FTIR) spectroscopy, x-ray microanalysis, compensated polarized light microscopy, and biochemical studies including 14C-labeled UDPG pyrophosphohydrolase radiometric assay.

RESULTS: This fraction of articular cartilage digest contained membrane-limited vesicles resembling growth plate cartilage matrix vesicles and formed mineral after only 24 hours in physiologic salt solution containing 1 mM ATP: The mineral contained inorganic pyrophosphate, 95% of which derived from ATP, and phosphate, 93% of which derived from inorganic phosphate in the medium. The FTIR spectrum of this mineral closely resembled the spectrum of standard calcium pyrophosphate dihydrate (CPPD) crystals. Compensated polarized light microscopy showed positively birefringent, rod-shaped crystals morphologically identical to CPPD. Ca:P ratios, defined by energy-dispersive microanalysis, were also consistent with CPPD.

CONCLUSION: The articular cartilage vesicle fraction of porcine hyaline cartilage is capable of generating mineral that strongly resembles CPPD.

Author List

Derfus BA, Rachow JW, Mandel NS, Boskey AL, Buday M, Kushnaryov VM, Ryan LM

Authors

Neil S. Mandel PhD Emeritus Professor in the Medicine department at Medical College of Wisconsin
Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adenosine Triphosphate
Adsorption
Animals
Calcium Pyrophosphate
Cartilage, Articular
Crystallization
Electron Probe Microanalysis
In Vitro Techniques
Microscopy, Electron
Spectrophotometry, Infrared
Swine