Interaction of von Willebrand factor with human platelets in the plasma milieu. J Clin Invest 1984 Feb;73(2):421-8
Date
02/01/1984Pubmed ID
6230372Pubmed Central ID
PMC425033DOI
10.1172/JCI111228Scopus ID
2-s2.0-0021347812 (requires institutional sign-in at Scopus site) 77 CitationsAbstract
The binding of von Willebrand factor (vWf) to stimulated platelets in the plasma milieu was performed using a radiolabeled monoclonal antibody to vWf. Plasma proteins specifically inhibited the thrombin- and ADP/epinephrine-induced vWf binding to activated platelets but did not inhibit the ristocetin-induced vWf binding. When normal plasma was heat defibrinated, monoclonal-labeled vWf was bound to platelets following thrombin or ADP/epinephrine stimulation. Furthermore, monoclonal-labeled vWf from afibrinogenemic plasma bound normally to platelets. The binding of vWf to stimulated platelets in either heat-defibrinated normal plasma or afibrinogenemic plasma was specifically inhibited by the addition of normal plasma fibrinogen in a concentration-dependent manner. At levels of fibrinogen less than 1 mg/ml, however, vWf binding could be demonstrated. The inhibition by fibrinogen of vWf binding to platelets was competitive and overcome by increased concentrations of vWf. These studies show that thrombin-induced and ADP/epinephrine-induced vWf binding to platelets does not occur in the plasma milieu, although at reduced levels of fibrinogen, vWf binding to stimulated platelets can be demonstrated.
Author List
Schullek J, Jordan J, Montgomery RRMESH terms used to index this publication - Major topics in bold
Adenosine DiphosphateAfibrinogenemia
Antibodies, Monoclonal
Blood Coagulation Factors
Blood Platelets
Blood Proteins
Epinephrine
Fibrinogen
Glycoproteins
Humans
Platelet Membrane Glycoproteins
Ristocetin
Thrombin
von Willebrand Diseases
von Willebrand Factor
