HNF-3/forkhead homologue-4 (HFH-4) is expressed in ciliated epithelial cells in the developing mouse lung. J Histochem Cytochem 1999 Jun;47(6):823-32
Date
05/20/1999Pubmed ID
10330459DOI
10.1177/002215549904700612Scopus ID
2-s2.0-0033023357 (requires institutional sign-in at Scopus site) 79 CitationsAbstract
HNF-3/forkhead homologue-4 (HFH-4), a transcription factor of the winged-helix/forkhead family, was detected by immunohistochemistry in tissue of the developing mouse. HFH-4 protein was present in epithelial cells of the lung, trachea, oviduct, and embryonic esophagus, and in ependymal cells lining the spinal column and ventricles of the brain. In lung, trachea, and nose, HFH-4 was expressed in a distinct subset of epithelial cells that also expressed beta-tubulin IV, a ciliated cell marker. Cellular sites of HFH-4 and beta-tubulin IV expression were distinct from that of Clara cell secretory protein (CCSP), which was detected in nonciliated epithelial cells in the conducting airway of the lung. HFH-4 and beta-tubulin IV, but not CCSP, were detected in the respiratory epithelium of thyroid transcription factor-1 (TTF-1) gene-targeted mice. The presence of HFH-4 and beta-tubulin IV in TTF-1 gene-targeted mice demonstrates that differentiation of ciliated epithelium does not require TTF-1. Co-localization of HFH-4 and beta-tubulin IV staining in various tissues during mouse development supports a role for HFH-4 in the differentiation of ciliated cell lineages.
Author List
Tichelaar JW, Wert SE, Costa RH, Kimura S, Whitsett JAMESH terms used to index this publication - Major topics in bold
AnimalsBasic Helix-Loop-Helix Leucine Zipper Transcription Factors
DNA-Binding Proteins
Epithelial Cells
Hepatocyte Nuclear Factor 4
Immunohistochemistry
Lung
Mice
Mice, Knockout
Nuclear Proteins
Phosphoproteins
Protein Biosynthesis
Thyroid Nuclear Factor 1
Tissue Distribution
Transcription Factors
Tubulin
Uteroglobin
