On the detection of paramagnetic species in the adriamycin-perfused rat heart: a reappraisal. Biochem Biophys Res Commun 1990 May 31;169(1):30-8
Date
05/31/1990Pubmed ID
2161659DOI
10.1016/0006-291x(90)91428-uScopus ID
2-s2.0-0025333513 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Recently, L. Costa et al. reported the direct detection of the superoxide anion and other paramagnetic species in the isolated, adriamycin-perfused rat heart [L. Costa et al. (1988) Biochem. Biophys. Res. Commun. 153, 275-280]. We have reevaluated the results of their study and concluded that the ESR parameters of the spectrum obtained from the adriamycin-perfused heart are consistent with that of the peroxyl radical and not with that of the superoxide anion. In addition, the ESR spectrum of the peroxyl radical is very likely produced as an artifact caused by the grinding of myocardial tissue. This artifact may mask the ESR spectra of the adriamycin-derived semiquinone radical and the iron-sulfur protein components of myocardium.
Author List
Kalyanaraman B, Baker JEAuthors
John E. Baker PhD Professor in the Surgery department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCoronary Disease
Doxorubicin
Electron Spin Resonance Spectroscopy
Free Radicals
Heart
In Vitro Techniques
Iron-Sulfur Proteins
Myocardium
Perfusion
Peroxides
Rats
Rats, Inbred Strains
Superoxides
