Identification of two borderline oxacillin-resistant strains of Staphylococcus aureus from routine nares swab specimens by one of three chromogenic agars evaluated for the detection of MRSA. Am J Clin Pathol 2010 Dec;134(6):921-7
Date
11/23/2010Pubmed ID
21088156DOI
10.1309/AJCPO9TOID1EPUIMScopus ID
2-s2.0-78649655793 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of nosocomial infections that result in extended hospital stays and increased mortality. Therefore, rapid, cost-effective techniques for surveillance and detection of MRSA are critical to the containment and prevention of the spread of MRSA within the health care environment. We examined the ability of 3 chromogenic media (Spectra MRSA, Remel, Lenexa, KS; MRSA Select, Bio-Rad, Redmond, WA; and ChromID MRSA, bioMerieux, Marcy l'Etoile, France) to detect MRSA from routine surveillance specimens following 18, 24, and 48 hours of incubation. Our results indicate that detection of MRSA using all 3 chromogenic media is optimal following 24 hours of incubation. Early examination reduced sensitivity, while extended incubation reduced specificity. In addition, Spectra MRSA identified 2 borderline oxacillin-resistant strains of S aureus that were not detected by the other 2 chromogenic agars evaluated. These strains demonstrate increased basal and inducible resistance to β-lactam antibiotics.
Author List
Buchan BW, Ledeboer NAAuthors
Blake W. Buchan PhD Professor in the Pathology department at Medical College of WisconsinNathan A. Ledeboer PhD Chief, Professor in the Pathology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AgarAnti-Bacterial Agents
Chromogenic Compounds
Cross Infection
Culture Media
Humans
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Nasal Mucosa
Oxacillin
Predictive Value of Tests
Staphylococcal Infections
Staphylococcus aureus
