Faculty Collaboration Database

Variations in site and levels of expression of chondrocyte nucleotide pyrophosphohydrolase with aging. J Bone Miner Res 2001 May;16(5):868-75

Date

05/09/2001

Pubmed ID

11341331

DOI

10.1359/jbmr.2001.16.5.868

Scopus ID

2-s2.0-0035055433 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

The aim of this study was to identify changes in cartilage intermediate layer protein/nucleotide pyrophosphohydrolase (CILP/NTPPH) expression in articular cartilage during aging. Adult (3-4 years old) and young (7-10 days old) porcine articular hyaline cartilage and fibrocartilage were studied by Northern blot analysis, in situ hybridization, and immunohistochemistry using a complementary DNA (cDNA) probe encoding porcine CILP/NTPPH and antibody to a synthetic peptide corresponding to a CILP/NTPPH sequence. Northern blot analysis of chondrocytes showed lower expression of CILP/NTPPH messenger RNA (mRNA) in young cartilage than in adult cartilage. In adult cartilage, extracellular matrix from the surface to the middeep zone was immunoreactive for CILP/NTPPH, especially in the pericellular matrix surrounding the middeep zone chondrocytes. In young cartilage, chondrocytes were moderately immunoreactive for CILP/NTPPH throughout all zones except the calcified zone. The matrix of young cartilage was negative except in the superficial zone. In young cartilage, CILP/NTPPH mRNA expression was undetectable. In adult cartilage, chondrocytes showed strong mRNA expression for CILP/NTPPH throughout middeep zones. Protein and mRNA signals were not detectable below the tidemark. CILP/NTPPH secretion into matrix around chondrocytes increases with aging. In this extracellular site it may generate inorganic pyrophosphate and contribute to age-related calcium pyrophosphate dihydrate crystal deposition disease.

Author List

Masuda I, Iyama KI, Halligan BD, Barbieri JT, Haas AL, McCarty DJ, Ryan LM

Authors

Joseph T. Barbieri PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin
Lawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Blotting, Northern
Cartilage, Articular
Chondrocytes
Extracellular Matrix Proteins
Gene Expression
Hyalin
Pyrophosphatases
Swine