Faculty Collaboration Database

Multiple signaling is involved in endostatin-mediated apoptosis in ECV 304 endothelial cells. Front Biosci 2005 May 01;10:1089-97

Date

03/17/2005

Pubmed ID

15769608

DOI

10.2741/1602

Scopus ID

2-s2.0-33745328553 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Apoptosis of vascular endothelial cells is associated with the regression of angiogenesis. Endostatin is a potential anti-angiogenic drug, but the effects of endostatin on apoptotic machinery in endothelial cells largely remain unclear. In the present study, human endostatin was expressed in E. Coli to induce apoptosis in endothelial cells. It was found that the expressed human endostatin specifically affected the viability of the ECV 304 in a dose-dependent manner. Endostatin induced apoptosis in these cells in a caspase-dependent manner, and endostatin-mediated apoptosis is associated with several apoptotic signaling pathways including overloading of intracellular magnesium and calcium, as well as regulation of p53 and Bcl 2 expression.

Author List

Ren B, Wang Y, Ndebele K, Zhi Q, Chen FH, Wang YZ, Parangi S

MESH terms used to index this publication - Major topics in bold

Apoptosis
Calcium
Caspase 3
Caspases
Endostatins
Endothelium, Vascular
Humans
Magnesium
Proto-Oncogene Proteins c-bcl-2
Signal Transduction
Tumor Suppressor Protein p53