Ischemia-induced changes in myocardial paramagnetic metabolites: implications for intracellular oxy-radical generation. FEBS Lett 1989 Feb 27;244(2):311-4
Date
02/27/1989Pubmed ID
2537756DOI
10.1016/0014-5793(89)80552-6Scopus ID
2-s2.0-0024561513 (requires institutional sign-in at Scopus site) 43 CitationsAbstract
Evidence to identify the cellular sources of oxy-radical generation in myocardium has been of an indirect nature. We have used low-temperature ESR spectroscopy to identify and characterize ischemia-induced changes in myocardial paramagnetic metabolites. Iron-sulfur proteins associated with the NADH or succinate dehydrogenases of the mitochondrial electron-transport chain were progressively reduced with the onset and development of ischemia. This study provides direct evidence for ischemia-induced changes in an intracellular source of superoxide radical generation that may contribute to oxy-radical production during reperfusion.
Author List
Baker JE, Kalyanaraman BAuthors
John E. Baker PhD Professor in the Surgery department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCoronary Disease
Electron Spin Resonance Spectroscopy
Free Radicals
In Vitro Techniques
Models, Biological
Myocardium
Perfusion
Rats
Rats, Inbred Strains