Augmentation of inorganic pyrophosphate elaboration in cartilage by serum factors. Arch Biochem Biophys 1989 Aug 01;272(2):386-92
Date
08/01/1989Pubmed ID
2546498DOI
10.1016/0003-9861(89)90232-4Scopus ID
2-s2.0-0024365598 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
The disordered production of inorganic pyrophosphate (PPi) by articular cartilage is thought to have an important role in the pathogenesis of calcium pyrophosphate dihydrate deposition disease and perhaps osteoarthritis. We have previously shown that fetal calf serum added to the culture media of porcine articular cartilage explants increases the elaboration of PPi into the ambient media. We have examined this PPi stimulatory activity by studying the effects of adult human serum (HS), serum derived from adult human plasma (HP), and an acid-alcohol extract of human platelets (PE) on PPi production in cartilage organ culture. Ten percent HS produces a 1.4-fold increase in PPi production after 48 h of culture, while cartilage incubated in media containing 10% HP produces no more PPi than that incubated in media alone. PE stimulates a mean 2-fold increase in PPi production at 48 h in the presence of low concentrations of HP, and has no effect alone. It does not appear to up-regulate the activity of the ectoenzyme nucleoside triphosphate pyrophosphohydrolase (NTPPPH), nor does it promote the release of enzyme substrate into the extracellular space. Cartilage exposed to 0.5% HP and PE has 1.51 +/- 0.36 units of NTPPPH activity whereas cartilage exposed to 0.5% HP alone has 1.52 +/- 0.41 units of enzyme activity. PE does not increase the release of [14C]adenine-labeled compounds into the media. Approximately 13% of soluble 14C counts was found in the media of chondrocytes treated with PE while 18% of counts was released in the presence of HP alone. We have demonstrated a factor or factors present in FCS, HS, and an acid-ethanol extract of human platelets which represent(s) the first known physiologic modulators of PPi production in articular cartilage and may increase PPi production without affecting NTPPPH activity.
Author List
Rosenthal AK, Cheung HS, Ryan LMAuthors
Ann K. Rosenthal MD Associate Dean, Professor in the Medicine department at Medical College of WisconsinLawrence M. Ryan MD Emeritus Professor in the Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AdenineAnimals
Blood Physiological Phenomena
Blood Platelets
Cartilage, Articular
Diphosphates
Humans
Organ Culture Techniques
Plasma
Pyrophosphatases
Swine
Thymidine
