Emerging technologies for the clinical microbiology laboratory. Clin Microbiol Rev 2014 Oct;27(4):783-822
Date
10/04/2014Pubmed ID
25278575Pubmed Central ID
PMC4187641DOI
10.1128/CMR.00003-14Scopus ID
2-s2.0-84907798849 (requires institutional sign-in at Scopus site) 252 CitationsAbstract
In this review we examine the literature related to emerging technologies that will help to reshape the clinical microbiology laboratory. These topics include nucleic acid amplification tests such as isothermal and point-of-care molecular diagnostics, multiplexed panels for syndromic diagnosis, digital PCR, next-generation sequencing, and automation of molecular tests. We also review matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry methods and their role in identification of microorganisms. Lastly, we review the shift to liquid-based microbiology and the integration of partial and full laboratory automation that are beginning to impact the clinical microbiology laboratory.
Author List
Buchan BW, Ledeboer NAAuthors
Blake W. Buchan PhD Professor in the Pathology and Laboratory Medicine department at Medical College of WisconsinNathan A. Ledeboer PhD Chief, Professor in the Pathology and Laboratory Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Automation, LaboratoryGenetic Techniques
Humans
Mass Spectrometry
Microbiological Techniques
