EGF is required for cardiac differentiation of P19CL6 cells through interaction with GATA-4 in a time- and dose-dependent manner. Cell Mol Life Sci 2015 May;72(10):2005-22
Date
12/17/2014Pubmed ID
25504289Pubmed Central ID
PMC11113121DOI
10.1007/s00018-014-1795-9Scopus ID
2-s2.0-84931834067 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
The regulation of cardiac differentiation is critical for maintaining normal cardiac development and function. The precise mechanisms whereby cardiac differentiation is regulated remain uncertain. Here, we have identified a GATA-4 target, EGF, which is essential for cardiogenesis and regulates cardiac differentiation in a dose- and time-dependent manner. Moreover, EGF demonstrates functional interaction with GATA-4 in inducing the cardiac differentiation of P19CL6 cells in a time- and dose-dependent manner. Biochemically, GATA-4 forms a complex with STAT3 to bind to the EGF promoter in response to EGF stimulation and cooperatively activate the EGF promoter. Functionally, the cooperation during EGF activation results in the subsequent activation of cyclin D1 expression, which partly accounts for the lack of additional induction of cardiac differentiation by the GATA-4/STAT3 complex. Thus, we propose a model in which the regulatory cascade of cardiac differentiation involves GATA-4, EGF, and cyclin D1.
Author List
Ma CX, Song YL, Xiao L, Xue LX, Li WJ, Laforest B, Komati H, Wang WP, Jia ZQ, Zhou CY, Zou Y, Nemer M, Zhang SF, Bai X, Wu H, Zang MXAuthor
Xiaowen Bai PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlotting, Western
Cell Differentiation
Cell Line, Tumor
Chromatin Immunoprecipitation
Epidermal Growth Factor
GATA4 Transcription Factor
Heart
Histological Techniques
Immunoprecipitation
Mice
Models, Biological
Myocardium
Real-Time Polymerase Chain Reaction
Signal Transduction
Time Factors
